Backbone 1H, 13C, and 15N chemical shift assignments of reduced horse heart cytochrome c
GDVEKGKKIF VQKCAQCHTV EKGGKHKTGP NLHGLFGRKT GQAPGFTYTD ANKNKGITWK EETLMEYLEN PKKYIPGTKM IFAGIKKKTE REDLIAYLKK ATNE
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:CYS14:SG | 2:HEC1:HEC |
2 | na | sing | 1:CYS17:SG | 2:HEC1:HEC |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 46.8 % (590 of 1260) | 25.9 % (173 of 667) | 64.5 % (312 of 484) | 96.3 % (105 of 109) |
Backbone | 81.5 % (502 of 616) | 52.3 % (113 of 216) | 97.3 % (292 of 300) | 97.0 % (97 of 100) |
Sidechain | 24.5 % (180 of 736) | 13.3 % (60 of 451) | 40.6 % (112 of 276) | 88.9 % (8 of 9) |
Aromatic | 8.3 % (8 of 96) | 10.4 % (5 of 48) | 6.4 % (3 of 47) | 0.0 % (0 of 1) |
Methyl | 14.1 % (13 of 92) | 6.5 % (3 of 46) | 21.7 % (10 of 46) |
1. hCc
GDVEKGKKIF VQKCAQCHTV EKGGKHKTGP NLHGLFGRKT GQAPGFTYTD ANKNKGITWK EETLMEYLEN PKKYIPGTKM IFAGIKKKTE REDLIAYLKK ATNESolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | hCc | [U-100% 13C; U-100% 15N] | 1 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 100 mM | |
4 | H2O | natural abundance | 95 % | |
5 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | hCc | [U-100% 13C; U-100% 15N] | 1 mM | |
7 | sodium phosphate | natural abundance | 20 mM | |
8 | H2O | natural abundance | 95 % | |
9 | D2O | natural abundance | 5 % |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 35.2 % (887 of 2520) | 23.4 % (312 of 1334) | 37.6 % (364 of 968) | 96.8 % (211 of 218) |
Backbone | 61.5 % (758 of 1232) | 51.9 % (224 of 432) | 56.5 % (339 of 600) | 97.5 % (195 of 200) |
Sidechain | 15.9 % (234 of 1472) | 9.9 % (89 of 902) | 23.4 % (129 of 552) | 88.9 % (16 of 18) |
Aromatic | 4.2 % (8 of 192) | 5.2 % (5 of 96) | 3.2 % (3 of 94) | 0.0 % (0 of 2) |
Methyl | 8.2 % (15 of 184) | 4.3 % (4 of 92) | 12.0 % (11 of 92) |
1. hCc
GDVEKGKKIF VQKCAQCHTV EKGGKHKTGP NLHGLFGRKT GQAPGFTYTD ANKNKGITWK EETLMEYLEN PKKYIPGTKM IFAGIKKKTE REDLIAYLKK ATNESolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | hCc | [U-100% 13C; U-100% 15N] | 1 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 100 mM | |
4 | H2O | natural abundance | 95 % | |
5 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | hCc | [U-100% 13C; U-100% 15N] | 1 mM | |
7 | sodium phosphate | natural abundance | 20 mM | |
8 | H2O | natural abundance | 95 % | |
9 | D2O | natural abundance | 5 % |