high resolution NMR solution structure of helix H1 of the human HAR1F RNA
Polymer type: polyribonucleotide
Total | 1H | |
---|---|---|
All | 75.0 % (243 of 324) | 75.0 % (243 of 324) |
Suger, PO4 | 64.4 % (143 of 222) | 64.4 % (143 of 222) |
Nucleobase | 98.0 % (100 of 102) | 98.0 % (100 of 102) |
Aromatic | 97.6 % (80 of 82) | 97.6 % (80 of 82) |
1. RNA (37-MER)
GGGUGAAACG GAGGACUUCG GUCCUCAAGU UUCACCCSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RNA (37-MER) | [U-100% 15N] | 1 mM | |
2 | potassium chloride | natural abundance | 50 mM | |
3 | potassium phosphate | natural abundance | 25 mM | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | RNA (37-MER) | [U-100% 15N] | 1 mM | |
7 | potassium chloride | natural abundance | 50 mM | |
8 | potassium phosphate | natural abundance | 25 mM | |
9 | D2O | natural abundance | 100 % |