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Mucin sequence based on MUC2 Mucin glycoprotein tandem repeat
Authors
Borgert, A., Heimburg-Molinaro, J., Lasanajak, Y., Ju, T., Liu, M., Thompson, P., Ragupathi, G., Barany, G., Cummings, R., Smith, D., Live, D.
Assembly
MUC2 Mucin Domain Peptide
Entity
1. MUC2 Mucin Domain Peptide (polymer, Thiol state: not present), 9 monomers, 781.948 Da Detail

XPTTTPLKX


Formula weight
781.948 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
torsion space simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 77.8 %, Completeness: 91.5 %, Completeness (bb): 81.6 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All91.5 % (75 of 82)100.0 % (43 of 43)79.4 % (27 of 34)100.0 % (5 of 5)
Backbone81.6 % (31 of 38)100.0 % (12 of 12)66.7 % (14 of 21)100.0 % (5 of 5)
Sidechain100.0 % (51 of 51)100.0 % (31 of 31)100.0 % (20 of 20)
Methyl100.0 % (10 of 10)100.0 % (5 of 5)100.0 % (5 of 5)

1. MUC2 Mucin Domain Peptide

XPTTTPLKX

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 4.5


#NameIsotope labelingTypeConcentration
1MUC2_Mucin_Domain_Peptidenatural abundance2.0 ~ 10.0 mM
2H2Onatural abundance90 %
3D2Onatural abundance10 %
Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 4.5


#NameIsotope labelingTypeConcentration
4MUC2_Mucin_Domain_Peptidenatural abundance2.0 ~ 10.0 mM
5D2Onatural abundance100 %

Protein Blocks Logo
Calculated from 67 models in PDB: 2LHV, Strand ID: A Detail


Release date
2012-03-26
Citation
Deciphering structural elements of mucin glycoprotein recognition
Borgert, A., Heimburg-Molinaro, J., Song, X., Lasanajak, Y., Ju, T., Liu, M., Thompson, P., Ragupathi, G., Barany, G., Smith, D.F., Cummings, R.D., Live, D.
ACS Chem. Biol. (2012), 7, 1031-1039, PubMed 22444368 , DOI 10.1021/cb300076s ,
Experiments performed 7 experiments Detail
NMR combined restraints 3 contents Detail
Keywords glycoprotein, glycosylation, MUC2, mucin, Tn antigen