Solution NMR structure of a DNA dodecamer containing the 7-aminomethyl-7-deaza-2'-deoxyguanosine adduct
Polymer type: polydeoxyribonucleotide
Total | 1H | |
---|---|---|
All | 56.9 % (62 of 109) | 56.9 % (62 of 109) |
Suger, PO4 | 55.8 % (43 of 77) | 55.8 % (43 of 77) |
Nucleobase | 59.4 % (19 of 32) | 59.4 % (19 of 32) |
Aromatic | 77.3 % (17 of 22) | 77.3 % (17 of 22) |
Methyl | 100.0 % (2 of 2) | 100.0 % (2 of 2) |
1. DNA dodecamer
GAGAXCGCTC TCSolvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DNA dodecamer | [U-2H] | 0.25 mM | |
2 | sodium phosphate | natural abundance | 10 mM | |
3 | sodium chloride | natural abundance | 200 mM | |
4 | sodium azide | natural abundance | 0.011 M | |
5 | sodium EDTA | natural abundance | 0.05 mM |