NMR structure of the lectin CCL2 (free)
MGHHHHHHHH SGDSPAVTLS AGNYIIYNRV LSPRGEKLAL TYPGRQRTPV TVSPLDGSSE QAWILRSYDS NSNTWTISPV GSPNSQIGWG AGNVPVVLPP NNYVWTLTLT SGGYNIQDGK RTVSWSLNNA TAGEEVSIGA DATFSGRWVI EKV
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 93.0 % (1579 of 1697) | 93.3 % (811 of 869) | 92.0 % (611 of 664) | 95.7 % (157 of 164) |
Backbone | 93.5 % (840 of 898) | 93.6 % (292 of 312) | 93.0 % (412 of 443) | 95.1 % (136 of 143) |
Sidechain | 93.1 % (871 of 936) | 93.2 % (519 of 557) | 92.5 % (331 of 358) | 100.0 % (21 of 21) |
Aromatic | 82.7 % (134 of 162) | 90.1 % (73 of 81) | 73.3 % (55 of 75) | 100.0 % (6 of 6) |
Methyl | 98.2 % (163 of 166) | 96.4 % (80 of 83) | 100.0 % (83 of 83) |
1. CCL2 (free)
MGHHHHHHHH SGDSPAVTLS AGNYIIYNRV LSPRGEKLAL TYPGRQRTPV TVSPLDGSSE QAWILRSYDS NSNTWTISPV GSPNSQIGWG AGNVPVVLPP NNYVWTLTLT SGGYNIQDGK RTVSWSLNNA TAGEEVSIGA DATFSGRWVI EKVSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | CCL2 | [U-100% 13C; U-100% 15N] | 1 mM | |
2 | potassium phosphate | natural abundance | 50 mM | |
3 | sodium chloride | natural abundance | 100 mM | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | CCL2 | [U-100% 13C; U-100% 15N] | 1 mM | |
7 | potassium phosphate | natural abundance | 50 mM | |
8 | sodium chloride | natural abundance | 100 mM | |
9 | D2O | natural abundance | 100 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | CCL2 | [U-100% 15N] | 1 mM | |
11 | potassium phosphate | natural abundance | 50 mM | |
12 | sodium chloride | natural abundance | 100 mM | |
13 | H2O | natural abundance | 90 % | |
14 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
15 | CCL2 | [U-100% 15N] | 1 mM | |
16 | potassium phosphate | natural abundance | 50 mM | |
17 | sodium chloride | natural abundance | 100 mM | |
18 | D2O | natural abundance | 100 % |