NOE-based 3D structure of the monomer of CylR2 in equilibrium with predissociated homodimer at 266K (-7 Celsius degrees)
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 76.8 % (646 of 841) | 80.9 % (360 of 445) | 66.7 % (214 of 321) | 96.0 % (72 of 75) |
Backbone | 73.1 % (285 of 390) | 89.2 % (116 of 130) | 54.8 % (108 of 197) | 96.8 % (61 of 63) |
Sidechain | 78.7 % (406 of 516) | 77.5 % (244 of 315) | 79.9 % (151 of 189) | 91.7 % (11 of 12) |
Aromatic | 87.0 % (40 of 46) | 87.0 % (20 of 23) | 90.9 % (20 of 22) | 0.0 % (0 of 1) |
Methyl | 97.7 % (86 of 88) | 97.7 % (43 of 44) | 97.7 % (43 of 44) |
1. CylR2
MIINNLKLIR EKKKISQSEL AALLEVSRQT INGIEKNKYN PSLQLALKIA YYLNTPLEDI FQWQPESolvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 266 (±0.2) K, pH 7.0 (±0.02)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | CylR2 | [U-100% 13C; U-100% 15N] | 0.52 (±0.05) mM | |
2 | sodium chloride | natural abundance | 600 mM | |
3 | HEPES | natural abundance | 50 mM | |
4 | H2O | natural abundance | 93 % | |
5 | D2O | natural abundance | 7 % |