NOE-based 3D structure of the monomeric intermediate of CylR2 at 262K (-11 Celsius degrees)
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 68.5 % (576 of 841) | 72.1 % (321 of 445) | 57.9 % (186 of 321) | 92.0 % (69 of 75) |
Backbone | 67.4 % (263 of 390) | 84.6 % (110 of 130) | 47.7 % (94 of 197) | 93.7 % (59 of 63) |
Sidechain | 68.0 % (351 of 516) | 67.0 % (211 of 315) | 68.8 % (130 of 189) | 83.3 % (10 of 12) |
Aromatic | 43.5 % (20 of 46) | 43.5 % (10 of 23) | 45.5 % (10 of 22) | 0.0 % (0 of 1) |
Methyl | 100.0 % (88 of 88) | 100.0 % (44 of 44) | 100.0 % (44 of 44) |
1. CylR2
MIINNLKLIR EKKKISQSEL AALLEVSRQT INGIEKNKYN PSLQLALKIA YYLNTPLEDI FQWQPESolvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 262 K, pH 7.0, Details sample was in 10 capillaries ca. 25-30 uL, each inserted into the regular NMR tube
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | CylR2 | [U-100% 13C; U-100% 15N] | 0.52 mM | |
2 | sodium chloride | natural abundance | 600 mM | |
3 | HEPES | natural abundance | 50 mM | |
4 | H2O | natural abundance | 93 % | |
5 | D2O | natural abundance | 7 % |