NOE-based 3D structure of the monomeric partially-folded intermediate of CylR2 at 257K (-16 Celsius degrees)
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 74.9 % (630 of 841) | 79.6 % (354 of 445) | 64.2 % (206 of 321) | 93.3 % (70 of 75) |
Backbone | 72.6 % (283 of 390) | 88.5 % (115 of 130) | 54.8 % (108 of 197) | 95.2 % (60 of 63) |
Sidechain | 76.2 % (393 of 516) | 75.9 % (239 of 315) | 76.2 % (144 of 189) | 83.3 % (10 of 12) |
Aromatic | 43.5 % (20 of 46) | 43.5 % (10 of 23) | 45.5 % (10 of 22) | 0.0 % (0 of 1) |
Methyl | 100.0 % (88 of 88) | 100.0 % (44 of 44) | 100.0 % (44 of 44) |
1. CylR2
MIINNLKLIR EKKKISQSEL AALLEVSRQT INGIEKNKYN PSLQLALKIA YYLNTPLEDI FQWQPESolvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 257 (±0.2) K, pH 7.0 (±0.02), Details sample was in 10 capillaries ca. 25-30 uL, each inserted into a 5 mm NMR tube
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | CylR2 | [U-100% 13C; U-100% 15N] | 0.52 (±0.05) mM | |
2 | sodium chloride | natural abundance | 600 mM | |
3 | HEPES | natural abundance | 50 mM | |
4 | H2O | natural abundance | 93 % | |
5 | D2O | natural abundance | 7 % |