1H, 13C and 15N NMR assignments of the aciniform spidroin (AcSp1) repetitive domain of Argiope trifasciata wrapping silk
AGPQGGFGAT GGASAGLISR VANALANTST LRTVLRTGVS QQIASSVVQR AAQSLASTLG VDGNNLARFA VQAVSRLPAG SDTSAYAQAF SSALFNAGVL NASNIDTLGS RVLSALLNGV SSAAQGLGIN VDSGSVQSDI SSSSSFLSTS SSSASYSQAS ASSTSGAGYT GPSGPSTGPS GYPGPLGGGA PFGQSGFGG
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 91.2 % (1788 of 1960) | 90.7 % (895 of 987) | 90.7 % (691 of 762) | 95.7 % (202 of 211) |
Backbone | 98.5 % (1160 of 1178) | 97.6 % (411 of 421) | 99.1 % (561 of 566) | 98.4 % (188 of 191) |
Sidechain | 83.6 % (794 of 950) | 85.5 % (484 of 566) | 81.3 % (296 of 364) | 70.0 % (14 of 20) |
Aromatic | 12.7 % (13 of 102) | 19.6 % (10 of 51) | 5.9 % (3 of 51) | |
Methyl | 96.4 % (214 of 222) | 98.2 % (109 of 111) | 94.6 % (105 of 111) |
1. W1
AGPQGGFGAT GGASAGLISR VANALANTST LRTVLRTGVS QQIASSVVQR AAQSLASTLG VDGNNLARFA VQAVSRLPAG SDTSAYAQAF SSALFNAGVL NASNIDTLGS RVLSALLNGV SSAAQGLGIN VDSGSVQSDI SSSSSFLSTS SSSASYSQAS ASSTSGAGYT GPSGPSTGPS GYPGPLGGGA PFGQSGFGGSolvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 303.150 K, pH 5.020
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | W1 | [U-13C; U-15N] | 0.76 (±5.0E-5) mM | |
2 | Acetate Buffer | natural abundance | 20 mM | |
3 | NaN3 | natural abundance | 1 mM | |
4 | DSS | natural abundance | 1 mM |