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BMRB: 17935

2LXN

1H, 13C, 15N assignment and secondary structure determination of GATase subunit of GMP Synthetase from Methanococcus jannaschii

Authors

Ali, R., Sarma, S.P.

Assembly

GATase subunit (monomer) of GMP Synthetase

Entity

1. GATase subunit (monomer) of GMP Synthetase (polymer, Thiol state: all free), 188 monomers, 21019.97 Da Detail

MIVILDNGGQ YVHRIHRSLK YIGVSSKIVP NTTPLEEIES NKEVKGIILS GGPDIEKAKN CIDIALNAKL PILGICLGHQ LIALAYGGEV GRAEAEEYAL TKVYVDKEND LFKNVPREFN AWASHKDEVK KVPEGFEILA HSDICQVEAM KHKTKPIYGV QFHPEVAHTE YGNEILKNFC KVCGYKFE

Formula weight

21019.97 Da

Source organism

Methanocaldococcus jannaschii

Exptl. method

solution NMR

Refine. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 62.0 %, Completeness (bb): 82.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	62.0 % (1378 of 2223)	63.8 % (738 of 1157)	52.2 % (455 of 871)	94.9 % (185 of 195)
Backbone	82.4 % (916 of 1112)	91.9 % (352 of 383)	70.7 % (388 of 549)	97.8 % (176 of 180)
Sidechain	49.2 % (632 of 1284)	50.0 % (387 of 774)	47.7 % (236 of 495)	60.0 % (9 of 15)
Aromatic	23.2 % (39 of 168)	42.9 % (36 of 84)	2.4 % (2 of 83)	100.0 % (1 of 1)
Methyl	59.8 % (134 of 224)	70.5 % (79 of 112)	49.1 % (55 of 112)

1. GATase subunit

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	GATase subunit	[U-99% 15N]	0.7 mM
2	D2O	natural abundance	10 %
3	H2O	natural abundance	90 %
4	PMSF	natural abundance	1 mM
5	potassium phosphate	natural abundance	20 mM
6	EDTA	natural abundance	0.1 mM
7	DTT	natural abundance	2 mM
8	sodium azide	natural abundance	0.01 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
9	GATase subunit	[U-99% 13C; U-99% 15N]	0.6 mM
10	H2O	natural abundance	90 %
11	D2O	natural abundance	10 %
12	potassium phosphate	natural abundance	20 mM
13	EDTA	natural abundance	0.1 mM
14	DTT	natural abundance	2 mM
15	PMSF	natural abundance	1 mM
16	sodium azide	natural abundance	0.01 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	indirect	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	indirect	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	indirect	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LXN, Strand ID: A Detail

Release date

2011-12-19

Citation

1H, 13C, 15N assignment and secondary structure determination of glutamine amido transferase subunit of gaunosine monophosphate synthetase from Methanocaldococcus jannaschii
Ali, R., Kumar, S., Balaram, H., Sarma, S.P.
Biomol. NMR Assign. (2012), 6, 193-196, PubMed 22203461 , DOI 10.1007/s12104-011-9354-x , Abstract

Related entities 1. GATase subunit (monomer) of GMP Synthetase, : 1 : 2 : 6 : 193 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC