NMR structure of Hsp12 in the presence of DPC
MSDAGRKGFG EKASEALKPD SQKSYAEQGK EYITDKADKV AGKVQPEDNK GVFQGVHDSA EKGKDNAEGQ GESLADQARD YMGAAKSKLN DAVEYVSGRV HGEEDPTKK
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 68.2 % (816 of 1196) | 75.3 % (475 of 631) | 52.7 % (237 of 450) | 90.4 % (104 of 115) |
Backbone | 80.7 % (523 of 648) | 97.8 % (223 of 228) | 62.7 % (197 of 314) | 97.2 % (103 of 106) |
Sidechain | 59.3 % (382 of 644) | 62.5 % (252 of 403) | 55.6 % (129 of 232) | 11.1 % (1 of 9) |
Aromatic | 0.0 % (0 of 60) | 0.0 % (0 of 30) | 0.0 % (0 of 30) | |
Methyl | 94.6 % (70 of 74) | 94.6 % (35 of 37) | 94.6 % (35 of 37) |
1. Hsp12
MSDAGRKGFG EKASEALKPD SQKSYAEQGK EYITDKADKV AGKVQPEDNK GVFQGVHDSA EKGKDNAEGQ GESLADQARD YMGAAKSKLN DAVEYVSGRV HGEEDPTKKSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.9, Details 200 mM DPC added
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Hsp12 | [U-13C; U-15N] | 0.7 mM | |
2 | DPC | natural abundance | 200 mM | |
3 | H2O | natural abundance | 90 % | |
4 | D2O | natural abundance | 10 % |