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BMRB: 18001

2LKN

1H, 13C and 15N chemical shift assignments of the PPIase domain of human aryl-hydrocarbon receptor-interacting protein (AIP)

Authors

Linnert, M., Haupt, K., Lin, Y., Kissing, S., Paschke, A., Fischer, G., Weiwad, M., Luecke, C.

Assembly

AIP

Entity

1. AIP (polymer, Thiol state: all free), 165 monomers, 18647.15 Da Detail

ADIIARLRED GIQKRVIQEG RGELPDFQDG TKATFHYRTL HSDDEGTVLD DSRARGKPME LIIGKKFKLP VWETIVCTMR EGEIAQFLCD IKHVVLYPLV AKSLRNIAVG KDPLEGQRHC CGVAQMREHS SLGHADLDAL QQNPQPLIFH MEMLKVESPG TYQQD

Formula weight

18647.15 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.4 %, Completeness (bb): 94.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.4 % (1860 of 1929)	96.7 % (978 of 1011)	96.7 % (722 of 747)	93.6 % (160 of 171)
Backbone	94.8 % (923 of 974)	94.6 % (317 of 335)	95.4 % (460 of 482)	93.0 % (146 of 157)
Sidechain	98.0 % (1085 of 1107)	97.8 % (661 of 676)	98.3 % (410 of 417)	100.0 % (14 of 14)
Aromatic	100.0 % (114 of 114)	100.0 % (57 of 57)	100.0 % (56 of 56)	100.0 % (1 of 1)
Methyl	98.9 % (180 of 182)	98.9 % (90 of 91)	98.9 % (90 of 91)

1. AIP

ADIIARLRED GIQKRVIQEG RGELPDFQDG TKATFHYRTL HSDDEGTVLD DSRARGKPME LIIGKKFKLP VWETIVCTMR EGEIAQFLCD IKHVVLYPLV AKSLRNIAVG KDPLEGQRHC CGVAQMREHS SLGHADLDAL QQNPQPLIFH MEMLKVESPG TYQQD

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	AIP	natural abundance	2 mM
2	sodium phosphate	natural abundance	10 mM
3	DTT	natural abundance	5 mM
4	sodium azide	natural abundance	0.05 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
7	AIP	[U-15N]	2 mM
8	sodium phosphate	natural abundance	10 mM
9	DTT	natural abundance	5 mM
10	sodium azide	natural abundance	0.05 mM
11	H2O	natural abundance	95 %
12	D2O	natural abundance	5 %

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
13	AIP	[U-13C; U-15N]	2 mM
14	sodium phosphate	natural abundance	10 mM
15	DTT	natural abundance	5 mM
16	sodium azide	natural abundance	0.05 mM
17	H2O	natural abundance	95 %
18	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LKN, Strand ID: A Detail

Release date

2012-01-31

Citation

NMR assignments of the FKBP-type PPIase domain of the human aryl-hydrocarbon receptor-interacting protein (AIP)
Linnert, M., Haupt, K., Lin, Y., Kissing, S., Paschke, A., Fischer, G., Weiwad, M., Luecke, C.
Biomol. NMR Assign. (2012), 6, 209-212, PubMed 22287093 , DOI 10.1007/s12104-012-9359-0 , Abstract

Related entities 1. AIP, : 1 : 2 : 30 entities Detail

Interaction partners 1. AIP, : 28 interactors Detail

More details for 28 interactors identified by 17 citations

Experiments performed 16 experiments Detail

1 2D 1H-1H TOCSY