BMRB: 18008

TBA

Authors

Tripsianes, K., Madl, T., Machyna, M., Fessas, D., Englbrecht, C., Neugebauer, U., Sattler, M.

Assembly

Molecular system

Entity

1. A (polymer, Thiol state: all free), 64 monomers, 6931.477 Da Detail

ASTQPTHSWK VGDKCMAVWS EDGQCYEAEI EEIDEENGTA AITFAGYGNA EVTPLLNLKP VEEG

2. DA2 (non-polymer), 202.254 Da

Total weight

7133.731 Da

Max. entity weight

6931.477 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 91.1 %, Completeness (bb): 84.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.1 % (633 of 695)	99.7 % (355 of 356)	77.9 % (211 of 271)	98.5 % (67 of 68)
Backbone	84.4 % (319 of 378)	99.2 % (130 of 131)	69.4 % (129 of 186)	98.4 % (60 of 61)
Sidechain	98.9 % (371 of 375)	100.0 % (225 of 225)	97.2 % (139 of 143)	100.0 % (7 of 7)
Aromatic	100.0 % (54 of 54)	100.0 % (27 of 27)	100.0 % (25 of 25)	100.0 % (2 of 2)
Methyl	100.0 % (64 of 64)	100.0 % (32 of 32)	100.0 % (32 of 32)

1. A

ASTQPTHSWK VGDKCMAVWS EDGQCYEAEI EEIDEENGTA AITFAGYGNA EVTPLLNLKP VEEG

Show sample condition

Sample

Solvent system 93% WATER/7% D2O, Pressure 1.000 atm, Temperature 298 K, pH 6.5, Details 4mM SPF30 18mM Da2

#	Name	Isotope labeling	Type	Concentration
1	SPF30	[U-13C; U-15N]		4 mM
2	DA2	natural abundance		18 mM
3	H2O	natural abundance		93 %
4	D2O	natural abundance		7 %
5	phosphate	natural abundance		20 mM
6	NaCl	natural abundance		50 mM

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LACS Plot; CA

Referencing offset: -0.17 ppm, Outliers: 5 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.17 ppm, Outliers: 5 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 4A4H, Strand ID: A Detail

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Release date

2011-11-01

Citation

Structural basis for dimethylarginine recognition by the Tudor domains of human SMN and SPF30 proteins
Tripsianes, K., Madl, T., Machyna, M., Fessas, D., Englbrecht, C., Fischer, U., Neugebauer, K., Sattler, M.
Nat. Struct. Mol. Biol. (2011), 18, 1414-1420, PubMed 22101937 , DOI 10.1038/nsmb.2185 , Abstract

Related entities 1. A, : 1 : 4 : 2 : 4 : 100 entities Detail

Interaction partners 1. A, : 32 interactors Detail

Experiments performed 6 experiments Detail