FF11-60
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 96.9 % (601 of 620) | 97.3 % (324 of 333) | 96.6 % (224 of 232) | 96.4 % (53 of 55) |
Backbone | 97.6 % (289 of 296) | 99.0 % (98 of 99) | 96.6 % (144 of 149) | 97.9 % (47 of 48) |
Sidechain | 96.8 % (361 of 373) | 96.6 % (226 of 234) | 97.7 % (129 of 132) | 85.7 % (6 of 7) |
Aromatic | 73.3 % (22 of 30) | 73.3 % (11 of 15) | 78.6 % (11 of 14) | 0.0 % (0 of 1) |
Methyl | 100.0 % (42 of 42) | 100.0 % (21 of 21) | 100.0 % (21 of 21) |
1. FF11-60
GNTKEEAKQA FKELLKEKRV PSNASWEQAM KMIINDPRYS ALAKLSEKKQSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.8, Details FF11-60
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | sodium acetate | natural abundance | 20 mM | |
2 | sodium chloride | natural abundance | 50 mM | |
3 | sodium azide | natural abundance | 0.05 % | |
4 | FF11-60 | [U-100% 13C; U-100% 15N] | 1 mM | |
5 | H2O | natural abundance | 95 % | |
6 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.8, Details FF11-60 A51L mutant to remove Ala chemical shift ambiguity
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | sodium acetate | natural abundance | 20 mM | |
8 | sodium chloride | natural abundance | 50 mM | |
9 | sodium azide | natural abundance | 0.05 % | |
10 | FF11-60 A51L | [U-100% 13C; U-100% 15N] | 1 mM | |
11 | H2O | natural abundance | 95 % | |
12 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.8, Details FF11-60 10% 13C-labelled sample for stereospecific methyl assignment experiment
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | 20mM sodium acetate | natural abundance | 20 mM | |
14 | 50 mM sodium chloride | natural abundance | 50 mM | |
15 | 0.05% sodium azide | natural abundance | 0.05 % | |
16 | FF11-60 | [U-10% 13C; U-100% 15N] | 1 mM | |
17 | H2O | natural abundance | 95 % | |
18 | D2O | natural abundance | 5 % |