Chemical shift assignments for the homeodomain of Pitx2 in complex with a TAATCC DNA binding site
Polymer type: polypeptide(L) polydeoxyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 72.1 % (776 of 1076) | 67.6 % (455 of 673) | 74.7 % (242 of 324) | 100.0 % (79 of 79) |
Backbone | 60.5 % (339 of 560) | 46.6 % (135 of 290) | 67.5 % (137 of 203) | 100.0 % (67 of 67) |
Sidechain | 85.4 % (498 of 583) | 83.6 % (320 of 383) | 88.3 % (166 of 188) | 100.0 % (12 of 12) |
Aromatic | 59.1 % (78 of 132) | 50.0 % (44 of 88) | 75.6 % (31 of 41) | 100.0 % (3 of 3) |
Methyl | 92.1 % (58 of 63) | 85.3 % (29 of 34) | 100.0 % (29 of 29) |
1. PITX2
GSQRRQRTHF TSQQLQELEA TFQRNRYPDM STREEIAVWT NLTEARVRVW FKNRRAKWRK REEFIVTD2. DNA 1
CTCTAATCCC C3. DNA 2
GGGGATTAGA GSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 (±0.1) K, pH 7.0 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | PITX2 | [U-99% 13C; U-99% 15N] | 1 mM | |
2 | DNA_1 | natural abundance | 1 mM | |
3 | DNA_2 | natural abundance | 1 mM | |
4 | H2O | [U-99% 13C; U-99% 15N] | 90 % | |
5 | D2O | [U-99% 13C; U-99% 15N] | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 (±0.1) K, pH 7.0 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | PITX2 | [U-99% 15N] | 1 mM | |
7 | DNA_1 | natural abundance | 1 mM | |
8 | DNA_2 | natural abundance | 1 mM | |
9 | H2O | [U-99% 13C; U-99% 15N] | 90 % | |
10 | D2O | [U-99% 13C; U-99% 15N] | 10 % |