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Structure of EDC3:DCP2
Authors
Truffault, V.
Assembly
EDC3 DCP2
Entity
1. EDC3 (polymer, Thiol state: all free), 96 monomers, 10587.88 Da Detail

MGMSVADFYG SNVEVLLNND SKARGVITNF DSSNSILQLR LANDSTKSIV TKDIKDLRIL PKNEIMPKNG TKSPSTNSTK LKSAETYSSK NKWSMD


2. DCP2 (polymer, Thiol state: all free), 48 monomers, 4940.379 Da Detail

GATTKEKNIS VDVDADASSQ LLSLLKSSTA PSDLATPQPS TFPQPPVE


Total weight
15528.259 Da
Max. entity weight
10587.88 Da
Source organism
Escherichia coli
Exptl. method
solution NMR
Refine. method
XPLOR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 78.5 %, Completeness: 68.2 %, Completeness (bb): 74.2 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All68.2 % (1106 of 1622)65.7 % (555 of 845)71.2 % (446 of 626)69.5 % (105 of 151)
Backbone74.2 % (628 of 846)71.5 % (203 of 284)76.1 % (325 of 427)74.1 % (100 of 135)
Sidechain64.2 % (587 of 915)62.7 % (352 of 561)68.0 % (230 of 338)31.3 % (5 of 16)
Aromatic34.5 % (20 of 58)69.0 % (20 of 29) 0.0 % (0 of 28) 0.0 % (0 of 1)
Methyl90.3 % (139 of 154)88.3 % (68 of 77)92.2 % (71 of 77)

1. EDC3

MGMSVADFYG SNVEVLLNND SKARGVITNF DSSNSILQLR LANDSTKSIV TKDIKDLRIL PKNEIMPKNG TKSPSTNSTK LKSAETYSSK NKWSMD

2. DCP2

GATTKEKNIS VDVDADASSQ LLSLLKSSTA PSDLATPQPS TFPQPPVE

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5


#NameIsotope labelingTypeConcentration
1EDC3[U-100% 15N]0.7 mM
2DCP2[U-100% 15N]0.7 mM
3NaPO4natural abundance50 mM
4imidazolenatural abundance10 mM
5NaClnatural abundance150 mM

LACS Plot; CA
Referencing offset: -0.09 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.09 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.03 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.29 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 22 models in PDB: 4A54, Strand ID: A, B Detail


Release date
2012-01-22
Citation
The structural basis of Edc3- and Scd6-mediated activation of the Dcp1:Dcp2 mRNA decapping complex
Fromm, S.A., Truffault, V., Kamenz, J., Braun, J.E., Hoffmann, N.A., Izaurralde, E., Sprangers, R.
EMBO J. (2012), 31, 279-290, PubMed 22085934 , DOI 10.1038/emboj.2011.408 ,
Related entities 1. EDC3, : 1 : 1 : 2 : 1 entities Detail
Related entities 2. DCP2, : 1 : 2 : 4 entities Detail
Interaction partners 1. EDC3, : 1 : 1 interactors Detail
Interaction partners 2. DCP2, : 3 : 1 interactors Detail
Experiments performed 1 experiments Detail
NMR combined restraints 9 contents Detail