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BMRB: 18045

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1H,13C, and 15N chemical shift assignments for human endothelial monocyte-activating polypeptide II

Authors

Lozhko, D., Stanek, J., Kazimierczuk, K., Zawadzka-Kazimierczuk, A., Kozminski, W., Zhukov, I., Kornelyuk, O.

Assembly

EMAP2

Entity

1. EMAP2 (polymer, Thiol state: all free), 169 monomers, 18535.33 Da Detail

AMDSKPIDVS RLDLRIGCII TARKHPDADS LYVEEVDVGE IAPRTVVSGL VNHVPLEQMQ NRMVILLCNL KPAKMRGVLS QAMVMCASSP EKIEILAPPN GSVPGDRITF DAFPGEPDKE LNPKKKIWEQ IQPDLHTNDE CVATYKGVPF EVKGKGVCRA QTMSNSGIK

Formula weight

18535.33 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.2 %, Completeness: 83.6 %, Completeness (bb): 82.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.6 % (1623 of 1941)	90.1 % (917 of 1018)	74.0 % (558 of 754)	87.6 % (148 of 169)
Backbone	82.5 % (813 of 986)	95.8 % (321 of 335)	69.8 % (346 of 496)	94.2 % (146 of 155)
Sidechain	86.5 % (963 of 1113)	87.3 % (596 of 683)	87.7 % (365 of 416)	14.3 % (2 of 14)
Aromatic	14.3 % (10 of 70)	17.1 % (6 of 35)	8.8 % (3 of 34)	100.0 % (1 of 1)
Methyl	97.9 % (190 of 194)	96.9 % (94 of 97)	99.0 % (96 of 97)

1. emap II

AMDSKPIDVS RLDLRIGCII TARKHPDADS LYVEEVDVGE IAPRTVVSGL VNHVPLEQMQ NRMVILLCNL KPAKMRGVLS QAMVMCASSP EKIEILAPPN GSVPGDRITF DAFPGEPDKE LNPKKKIWEQ IQPDLHTNDE CVATYKGVPF EVKGKGVCRA QTMSNSGIK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±0.2) K, pH 8.0 (±0.3)

#	Name	Isotope labeling	Concentration
1	emap II	[U-98% 13C; U-98% 15N]	0.7 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	250 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.11 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.11 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

Release date

2012-03-08

Citation

(1)H, (13)C, and (15)N chemical shifts assignments for human endothelial monocyte-activating polypeptide EMAP II
Lozhko, D., Stanek, J., Kazimierczuk, K., Zawadzka-Kazimierczuk, A., Kozminski, W., Zhukov, I., Kornelyuk, A.
Biomol. NMR Assign. (2013), 7, 25-29, PubMed 22392337 , DOI 10.1007/s12104-012-9369-y , Abstract

Related entities 1. EMAP2, : 1 : 2 : 62 entities Detail

Interaction partners 1. EMAP2, : 39 interactors Detail

More details for 39 interactors identified by 14 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC