Solution NMR structure of the novel conotoxin im23a from Conus imperialis
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS4:SG | 1:CYS11:SG |
2 | disulfide | sing | 1:CYS15:SG | 1:CYS25:SG |
3 | disulfide | sing | 1:CYS26:SG | 1:CYS41:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 67.3 % (330 of 490) | 98.1 % (253 of 258) | 18.0 % (34 of 189) | 100.0 % (43 of 43) |
Backbone | 61.1 % (149 of 244) | 98.8 % (81 of 82) | 24.2 % (30 of 124) | 100.0 % (38 of 38) |
Sidechain | 63.6 % (182 of 286) | 97.7 % (172 of 176) | 4.8 % (5 of 105) | 100.0 % (5 of 5) |
Aromatic | 54.0 % (27 of 50) | 100.0 % (25 of 25) | 0.0 % (0 of 23) | 100.0 % (2 of 2) |
Methyl | 61.5 % (16 of 26) | 100.0 % (13 of 13) | 23.1 % (3 of 13) |
1. entity
IPYCGQTGAE CYSWCIKQDL SKDWCCDFVK DIRMNPPADK CPSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 5.6
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity | [U-15N] | 128 uM | |
2 | H2O | natural abundance | 95 % | |
3 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 5.6
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | entity | natural abundance | 128 uM | |
5 | H2O | natural abundance | 95 % | |
6 | D2O | natural abundance | 5 % |