Solution structure of Sgf73(59-102) zinc finger domain
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | covalent | sing | 1:CYS20:SG | 2:ZN1:ZN |
2 | covalent | sing | 1:CYS23:SG | 2:ZN1:ZN |
3 | covalent | sing | 1:HIS35:NE2 | 2:ZN1:ZN |
4 | covalent | sing | 1:HIS39:ND1 | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 85.6 % (423 of 494) | 82.9 % (214 of 258) | 89.0 % (170 of 191) | 86.7 % (39 of 45) |
Backbone | 94.1 % (241 of 256) | 91.9 % (79 of 86) | 95.4 % (124 of 130) | 95.0 % (38 of 40) |
Sidechain | 76.4 % (214 of 280) | 73.3 % (126 of 172) | 84.5 % (87 of 103) | 20.0 % (1 of 5) |
Aromatic | 18.8 % (3 of 16) | 37.5 % (3 of 8) | 0.0 % (0 of 8) | |
Methyl | 100.0 % (52 of 52) | 100.0 % (26 of 26) | 100.0 % (26 of 26) |
1. sgf73
NPNAQLIEDP LDKPIQYRVC EKCGKPLALT AIVDHLENHC AGASSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | sgf73 | [U-98% 15N] | 0.2 mM | |
2 | sodium phosphate | natural abundance | 50 mM | |
3 | sodium chloride | natural abundance | 100 mM | |
4 | TCEP | natural abundance | 0.05 mM | |
5 | D2O | natural abundance | 10 % | |
6 | H2O | natural abundance | 90 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | sgf73 | [U-99% 13C; U-98% 15N] | 1 mM | |
8 | sodium phosphate | natural abundance | 20 mM | |
9 | sodium chloride | natural abundance | 75 mM | |
10 | DTT | natural abundance | 1 mM | |
11 | D2O | natural abundance | 10 % | |
12 | H2O | natural abundance | 90 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | sgf73 | [U-98% 15N] | 0.2 mM | |
14 | sodium phosphate | natural abundance | 50 mM | |
15 | sodium chloride | natural abundance | 100 mM | |
16 | TCEP | natural abundance | 0.05 mM | |
17 | D2O | natural abundance | 100 % |