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BMRB: 18211

2LY2

1H, 15N and 13C resonance assignments for the LOTUS2 and LOTUS3 RNA binding domains of mouse TDRD7

Authors

Cui, G., Botuyan, M., Mer, G.

Assembly

Lotus domains 2 and 3

Entity

1. Lotus domains 2 and 3 (polymer, Thiol state: all free), 180 monomers, 20294.92 Da Detail

GHMDEVQNRI KEILDKHNNG IWISKLPHFY KEFYKEDLNQ GVLQQFEHWP HICTVEKPCG GGQDSLLYPA RREQPLKSDQ DPEKELPPPP PAPKQEVPSQ GSPAVMPDVK EKVAELLGKY SSGLWASALP KAFEDMYKVK FPEDALKNLA SLSDVCTINY ISGNTQKAIL YAKLPLPTDK

Formula weight

20294.92 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.5 %, Completeness (bb): 98.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	98.5 % (2132 of 2164)	98.0 % (1121 of 1144)	99.2 % (832 of 839)	98.9 % (179 of 181)
Backbone	98.3 % (1024 of 1042)	97.4 % (342 of 351)	98.7 % (523 of 530)	98.8 % (159 of 161)
Sidechain	98.8 % (1277 of 1292)	98.2 % (779 of 793)	99.8 % (478 of 479)	100.0 % (20 of 20)
Aromatic	100.0 % (162 of 162)	100.0 % (81 of 81)	100.0 % (78 of 78)	100.0 % (3 of 3)
Methyl	99.4 % (169 of 170)	98.8 % (84 of 85)	100.0 % (85 of 85)

1. TDRD7

GHMDEVQNRI KEILDKHNNG IWISKLPHFY KEFYKEDLNQ GVLQQFEHWP HICTVEKPCG GGQDSLLYPA RREQPLKSDQ DPEKELPPPP PAPKQEVPSQ GSPAVMPDVK EKVAELLGKY SSGLWASALP KAFEDMYKVK FPEDALKNLA SLSDVCTINY ISGNTQKAIL YAKLPLPTDK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	TDRD7 (LOTUS1-LOTUS2)	[U-100% 13C; U-100% 15N]	1 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.14 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LY2, Strand ID: A Detail

Release date

2012-03-19

Citation

(1)H, (15)N and (13)C resonance assignments for the three LOTUS RNA binding domains of Tudor domain-containing protein TDRD7
Cui, G., Botuyan, M., Mer, G.
Biomol. NMR Assign. (2013), 7, 79-83, PubMed 22481467 , DOI 10.1007/s12104-012-9382-1 , Abstract

Related entities 1. Lotus domains 2 and 3, : 1 : 1 : 2 : 19 entities Detail

Interaction partners 1. Lotus domains 2 and 3, : 17 interactors Detail

More details for 17 interactors identified by 5 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC