Backbone structure of human membrane protein HIGD1A
MSTDTGVSLP SYEEDQGSKL IRKAKEAPFV PVGIAGFAAI VAYGLYKLKS RGNTKMSIHL IHMRVAAQGF VVGAMTVGMG YSMYREFWAK PKP
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 47.7 % (515 of 1079) | 39.2 % (219 of 559) | 52.3 % (224 of 428) | 78.3 % (72 of 92) |
Backbone | 72.4 % (397 of 548) | 67.0 % (128 of 191) | 73.2 % (197 of 269) | 81.8 % (72 of 88) |
Sidechain | 29.5 % (181 of 614) | 24.7 % (91 of 368) | 37.2 % (90 of 242) | 0.0 % (0 of 4) |
Aromatic | 2.0 % (2 of 100) | 4.0 % (2 of 50) | 0.0 % (0 of 49) | 0.0 % (0 of 1) |
Methyl | 52.0 % (52 of 100) | 46.0 % (23 of 50) | 58.0 % (29 of 50) |
1. entity
MSTDTGVSLP SYEEDQGSKL IRKAKEAPFV PVGIAGFAAI VAYGLYKLKS RGNTKMSIHL IHMRVAAQGF VVGAMTVGMG YSMYREFWAK PKPSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0, Details U-15N-labeled
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HIGD1A | [U-15N] | 0.2 mM | |
2 | MES-Bis-TRIS | natural abundance | 20 mM | |
3 | LMPG | natural abundance | 2 % | |
4 | H2O | natural abundance | 95 % | |
5 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0, Details U-15N,13C-labeled
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | HIGD1A | [U-15N; U-13C] | 0.2 mM | |
7 | MES-Bis-TRIS | natural abundance | 20 mM | |
8 | LMPG | natural abundance | 2 % | |
9 | H2O | natural abundance | 95 % | |
10 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0, Details U-15N,13C,2H-labeled
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | HIGD1A | [U-15N; U-13C; U-2H] | 0.2 mM | |
12 | MES-Bis-TRIS | natural abundance | 20 mM | |
13 | LMPG | natural abundance | 2 % | |
14 | H2O | natural abundance | 95 % | |
15 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0, Details Combinatorial residue-selective 15N,13C1-labeled
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
16 | HIGD1A | [U-15N; U-13C1] | 0.2 mM | |
17 | MES-Bis-TRIS | natural abundance | 20 mM | |
18 | LMPG | natural abundance | 2 % | |
19 | H2O | natural abundance | 95 % | |
20 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0, Details U-15N-labeled single-cysteine mutants with paramagnetic MTSL label
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
21 | HIGD1A | [U-15N] | 0.2 mM | |
22 | MES-Bis-TRIS | natural abundance | 20 mM | |
23 | LMPG | natural abundance | 2 % | |
24 | H2O | natural abundance | 95 % | |
25 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0, Details U-15N-labeled single-cysteine mutants with diamagnetic label (MTSL analog)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
26 | HIGD1A | [U-15N] | 0.2 mM | |
27 | MES-Bis-TRIS | natural abundance | 20 mM | |
28 | LMPG | natural abundance | 2 % | |
29 | H2O | natural abundance | 95 % | |
30 | D2O | natural abundance | 5 % |