Backbone structure of human membrane protein TMEM14A from NOE data
MTSLYKKVGM DLIGFGYAAL VTFGSIFGYK RRGGVPSLIA GLFVGCLAGY GAYRVSNDKR DVKVSLFTAF FLATIMGVRF KRSKKIMPAG LVAGLSLMMI LRLVLLLL
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 61.4 % (787 of 1281) | 50.7 % (333 of 657) | 69.1 % (357 of 517) | 90.7 % (97 of 107) |
Backbone | 86.2 % (555 of 644) | 86.0 % (196 of 228) | 84.8 % (263 of 310) | 90.6 % (96 of 106) |
Sidechain | 43.0 % (314 of 731) | 31.9 % (137 of 429) | 58.5 % (176 of 301) | 100.0 % (1 of 1) |
Aromatic | 6.7 % (8 of 120) | 13.3 % (8 of 60) | 0.0 % (0 of 60) | |
Methyl | 59.5 % (94 of 158) | 45.6 % (36 of 79) | 73.4 % (58 of 79) |
1. entity
MTSLYKKVGM DLIGFGYAAL VTFGSIFGYK RRGGVPSLIA GLFVGCLAGY GAYRVSNDKR DVKVSLFTAF FLATIMGVRF KRSKKIMPAG LVAGLSLMMI LRLVLLLLSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0, Details U-15N-labeled protein
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TMEM14A | [U-15N] | 0.2 mM | |
2 | MES-Bis-TRIS | natural abundance | 20 mM | |
3 | LMPG | natural abundance | 2 % | |
4 | DSS | natural abundance | 0.5 mM | |
5 | H2O | natural abundance | 95 % | |
6 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0, Details U-15N-13C-labeled protein
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | TMEM14A | [U-15N; U-13C] | 0.2 mM | |
8 | MES-Bis-TRIS | natural abundance | 20 mM | |
9 | LMPG | natural abundance | 2 % | |
10 | DSS | natural abundance | 0.5 mM | |
11 | H2O | natural abundance | 95 % | |
12 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0, Details U-15N-13C-2H-labeled protein
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | TMEM14A | [U-15N; U-13C; U-2H] | 0.2 mM | |
14 | MES-Bis-TRIS | natural abundance | 20 mM | |
15 | LMPG | natural abundance | 2 % | |
16 | DSS | natural abundance | 0.5 mM | |
17 | H2O | natural abundance | 95 % | |
18 | D2O | natural abundance | 5 % |