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AR55 solubilised in LPPG micelles
Authors
Langelaan, D.N., Rainey, J.K.
Assembly
AR55
Entity
1. AR55 (polymer, Thiol state: all free), 64 monomers, 7296.025 Da Detail

MEEGGDFDNY YGADNQSECE YTDWKSSGAL IPAIYMLVFL LGTTGNGLVL WTVFRKKGHH HHHH


Formula weight
7296.025 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
simulated annealing, torsion angle dynamics
Data set
assigned_chemical_shifts, spectral_peak_list
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 89.4 %, Completeness (bb): 96.9 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All89.4 % (661 of 739)86.5 % (326 of 377)91.1 % (267 of 293)98.6 % (68 of 69)
Backbone96.9 % (370 of 382)96.3 % (130 of 135)96.7 % (178 of 184)98.4 % (62 of 63)
Sidechain84.0 % (347 of 413)81.0 % (196 of 242)87.9 % (145 of 165)100.0 % (6 of 6)
Aromatic69.1 % (76 of 110)72.7 % (40 of 55)64.2 % (34 of 53)100.0 % (2 of 2)
Methyl96.6 % (56 of 58)96.6 % (28 of 29)96.6 % (28 of 29)

1. AR55

MEEGGDFDNY YGADNQSECE YTDWKSSGAL IPAIYMLVFL LGTTGNGLVL WTVFRKKGHH HHHH

Sample

Solvent system 95% H2O/5% D2O, Temperature 310 K, pH 5, Details AR55 solubilised in LPPG micelles


#NameIsotope labelingTypeConcentration
1AR55[U-99% 13C; U-99% 15N]0.56 mM
2LPPGnatural abundance72 mM
3sodium acetate[U-2H]20 mM
4sodium azidenatural abundance1 mM
5DSSnatural abundance1 mM
6DTT[U-99% 2H]10 mM
7H2Onatural abundance95 %
8D2Onatural abundance5 %

LACS Plot; CA
Referencing offset: -0.19 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.19 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.01 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.17 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 40 models in PDB: 2LOV, Strand ID: A Detail


Release date
2012-01-31
Citation
Preserved Transmembrane Segment Topology, Structure, and Dynamics in Disparate Micellar Environments
Langelaan, D.N., Pandey, A., Sarker, M., Rainey, J.K.
J. Phys. Chem. Lett. (2017), 8, 2381-2386, PubMed 28492329 , DOI 10.1021/acs.jpclett.7b00867 ,
Related entities 1. AR55, : 1 : 3 : 3 : 33 entities Detail
Interaction partners 1. AR55, : 2 interactors Detail
Experiments performed 15 experiments Detail
NMR combined restraints 3 contents Detail
Keywords LPPG micelles, apelin receptor