Mutant of the sub-genomic promoter from Brome Mosaic Virus
Polymer type: polyribonucleotide
Total | 1H | 31P | |
---|---|---|---|
All | 77.2 % (112 of 145) | 75.4 % (98 of 130) | 93.3 % (14 of 15) |
Suger, PO4 | 69.5 % (73 of 105) | 65.6 % (59 of 90) | 93.3 % (14 of 15) |
Nucleobase | 97.5 % (39 of 40) | 97.5 % (39 of 40) | |
Aromatic | 97.1 % (33 of 34) | 97.1 % (33 of 34) |
1. RNA (5'-R(*GP*AP*GP*GP*AP*CP*AP*UP*AP*GP*UP*CP*UP*UP*C)-3')
GAGGACAUAG UCUUCSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 2 mM RNA, 10 mM sodium phosphate buffer (pH 7), 50 mM NaCl, 2 mM EDTA
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | delA_RNA | natural abundance | 2 mM | |
2 | sodium phosphate | natural abundance | 10 mM | |
3 | sodium chloride | natural abundance | 50 mM | |
4 | EDTA | natural abundance | 2 mM | |
5 | H2O | natural abundance | 90 % | |
6 | D2O | natural abundance | 10 % |