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BMRB: 18282

2LPY

SOLUTION STRUCTURE OF THE M-PMV MYRISTOYLATED MATRIX PROTEIN

Authors

Prchal, J., Hrabal, R.

Assembly

M-PMV MYRISTOYLATED MATRIX PROTEIN

Entity

1. M-PMV MATRIX PROTEIN (polymer, Thiol state: all free), 124 monomers, 14689.43 Da Detail

GQELSQHERY VEQLKQALKT RGVKVKYADL LKFFDFVKDT CPWFPQEGTI DIKRWRRVGD CFQDYYNTFG PEKVPVTAFS YWNLIKELID KKEVNPQVMA AVAQTEEILK SNSQTDLEHH HHHH

2. MYR (non-polymer, Thiol state: not present), 228.371 Da

Total weight

14917.801 Da

Max. entity weight

14689.43 Da

Source organism

Mason-Pfizer monkey virus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 65.6 %, Completeness (bb): 87.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	65.6 % (1007 of 1535)	59.5 % (478 of 803)	71.2 % (425 of 597)	77.0 % (104 of 135)
Backbone	87.6 % (643 of 734)	87.9 % (218 of 248)	87.7 % (322 of 367)	86.6 % (103 of 119)
Sidechain	51.3 % (472 of 920)	46.8 % (260 of 555)	60.5 % (211 of 349)	6.3 % (1 of 16)
Aromatic	25.3 % (44 of 174)	49.4 % (43 of 87)	0.0 % (0 of 84)	33.3 % (1 of 3)
Methyl	73.8 % (90 of 122)	72.1 % (44 of 61)	75.4 % (46 of 61)

1. M-PMV MATRIX PROTEIN

GQELSQHERY VEQLKQALKT RGVKVKYADL LKFFDFVKDT CPWFPQEGTI DIKRWRRVGD CFQDYYNTFG PEKVPVTAFS YWNLIKELID KKEVNPQVMA AVAQTEEILK SNSQTDLEHH HHHH

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Concentration
1	potassium phosphate	natural abundance	100 mM
2	sodium chloride	natural abundance	300 mM
3	DTT	natural abundance	5 mM
4	M-PMV MATRIX PROTEIN	[U-99% 13C; U-99% 15N]	0.5 mM
5	H2O	natural abundance	90 %
6	D2)	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbon	external	direct	1.0
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.1013291

Referencing offset: 0.26 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbon	external	direct	1.0
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.1013291

Referencing offset: 0.26 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbon	external	direct	1.0
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.1013291

Referencing offset: 0.11 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbon	external	direct	1.0
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 19 models in PDB: 2LPY, Strand ID: A Detail

Release date

2012-08-20

Citation

The structure of myristoylated Mason-Pfizer monkey virus matrix protein and the role of phosphatidylinositol-(4,5)-bisphosphate in its membrane binding
Prchal, J., Srb, P., Hunter, E., Ruml, T., Hrabal, R.
J. Mol. Biol. (2012), 423, 427-438, PubMed 22863803 , DOI 10.1016/j.jmb.2012.07.021 , Abstract

Related entities 1. M-PMV MATRIX PROTEIN, : 1 : 1 : 2 : 21 entities Detail

Interaction partners 1. M-PMV MATRIX PROTEIN, : 1 interactors Detail

More details for 1 interactors identified by 1 citations