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BMRB: 18316

2LQJ

Solution structure of the C-terminal domain of the MgtC protein from Mycobacterium tuberculosis

Authors

Yang, M., Yang, Y., Labesse, G., Blanc-Potard, A.

Assembly

C-terminal domain of the MgtC protein

Entity

1. C-terminal domain of the MgtC protein (polymer, Thiol state: all free), 94 monomers, 10400.65 Da Detail

VEDEGLQPYQ VRVICRPKAE TYVRAHIVQR TSSNDITLRG IRTGPAGDDN ITLTAHLLMV GHTPAKLERL VAELSLQPGV YAVHWYAGEH AQAE

Formula weight

10400.65 Da

Source organism

Mycobacterium tuberculosis

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 56.3 %, Completeness (bb): 98.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	56.3 % (596 of 1059)	41.3 % (225 of 545)	67.6 % (282 of 417)	91.8 % (89 of 97)
Backbone	98.6 % (546 of 554)	100.0 % (190 of 190)	97.1 % (267 of 275)	100.0 % (89 of 89)
Sidechain	22.5 % (133 of 592)	9.9 % (35 of 355)	42.8 % (98 of 229)	0.0 % (0 of 8)
Aromatic	0.0 % (0 of 64)	0.0 % (0 of 32)	0.0 % (0 of 31)	0.0 % (0 of 1)
Methyl	24.6 % (31 of 126)	19.0 % (12 of 63)	30.2 % (19 of 63)

1. entity

VEDEGLQPYQ VRVICRPKAE TYVRAHIVQR TSSNDITLRG IRTGPAGDDN ITLTAHLLMV GHTPAKLERL VAELSLQPGV YAVHWYAGEH AQAE

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 4.6

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 15N]	0.3 mM
2	H20	natural abundance	90 %
3	D20	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 4.6

#	Name	Isotope labeling	Concentration
4	entity	[U-100% 13C; U-100% 15N]	0.3 mM
5	H20	natural abundance	90 %
6	D20	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.0
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.0

Referencing offset: 0.0 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.0
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.0

Referencing offset: 0.0 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.0
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.0

Referencing offset: -0.18 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.0
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.0

Referencing offset: 0.22 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 2LQJ, Strand ID: A Detail

Release date

2012-04-10

Citation

The C-terminal domain of the virulence factor MgtC is a divergent ACT domain
Yang, Y., Labesse, G., Carrere-Kremer, S., Esteves, K., Kremer, L., Cohen-Gonsaud, M., Blanc-Potard, A.
J. Bacteriol. (2012), 194, 6255-6263, PubMed 22984256 , DOI 10.1128/JB.01424-12 , Abstract

Related entities 1. C-terminal domain of the MgtC protein, : 1 : 1 : 7 entities Detail

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC