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Structure of the RNA claw of the DNA packaging motor of bacteriophage 29
Authors
Harjes, E.J., Matsuo, H.J., Kitamura, A.J.
Assembly
RNA (27-MER)
Entity
1. RNA (27-MER) (polymer, Thiol state: not present), 27 monomers, 8649.098 Da Detail

GGACUUCCAU UGCUUCGGCA AAAGUCC


Formula weight
8649.098 Da
Source organism
Staphylococcus virus 29
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 63.4 %, Completeness (bb): 53.2 % Detail

Polymer type: polyribonucleotide

Total1H13C15N
All63.4 % (276 of 435)85.8 % (205 of 239)37.7 % (69 of 183)15.4 % (2 of 13)
Suger, PO453.2 % (158 of 297)82.7 % (134 of 162)17.8 % (24 of 135)
Nucleobase85.5 % (118 of 138)92.2 % (71 of 77)93.8 % (45 of 48)15.4 % (2 of 13)
Aromatic86.9 % (106 of 122)96.7 % (59 of 61)93.8 % (45 of 48)15.4 % (2 of 13)

1. RNA (27-MER)

GGACUUCCAU UGCUUCGGCA AAAGUCC

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5


#NameIsotope labelingTypeConcentration
1RNA (27-MER)[U-100% 13C; U-100% 15N]300 uM
2H2Onatural abundance90 %
3D2Onatural abundance10 %

Release date
2012-07-29
Citation
Structure of the RNA claw of the DNA packaging motor of bacteriophage Φ29
Harjes, E., Kitamura, A., Zhao, W., Morais, M.C., Jardine, P.J., Grimes, S., Matsuo, H.
Nucleic Acids Res. (2012), 40, 9953-9963, PubMed 22879380 , DOI 10.1093/nar/gks724 ,
Related entities 1. RNA (27-MER), : 1 entities Detail
Experiments performed 5 experiments Detail
NMR combined restraints 10 contents Detail
Keywords bulge, RNA