C-terminal propeptide (PPc) region of vibrio extracellular metalloprotease
GNVLKNNTPV SNLTGNKGSE VFYTFTVDRN ATAVVSISGG SGDADLYLKA GSKPTTSSWD CRPYRYGNNE SCSVSAAPGT TYHVMIKGYS NYSGVTLKLQ Y
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 90.6 % (997 of 1100) | 93.1 % (523 of 562) | 86.5 % (372 of 430) | 94.4 % (102 of 108) |
Backbone | 96.7 % (578 of 598) | 95.7 % (200 of 209) | 97.3 % (284 of 292) | 96.9 % (94 of 97) |
Sidechain | 86.0 % (509 of 592) | 91.5 % (323 of 353) | 78.1 % (178 of 228) | 72.7 % (8 of 11) |
Aromatic | 34.0 % (34 of 100) | 62.0 % (31 of 50) | 4.1 % (2 of 49) | 100.0 % (1 of 1) |
Methyl | 98.0 % (98 of 100) | 98.0 % (49 of 50) | 98.0 % (49 of 50) |
1. C-terminal propeptide (PPc) region of vibrio extracellular metalloprotease
GNVLKNNTPV SNLTGNKGSE VFYTFTVDRN ATAVVSISGG SGDADLYLKA GSKPTTSSWD CRPYRYGNNE SCSVSAAPGT TYHVMIKGYS NYSGVTLKLQ YSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | PPc protein | [U-100% 15N] | 0.6 mM | |
2 | HEPES (pH 7.0) | natural abundance | 20 mM | |
3 | Sodium chloride | natural abundance | 100 mM | |
4 | DTT | natural abundance | 10 mM | |
5 | NaN3 | natural abundance | 0.01 % | |
6 | H2O | natural abundance | 90 % | |
7 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | PPc protein | [U-100% 13C; U-100% 15N] | 0.6 mM | |
9 | HEPES (pH 7.0) | natural abundance | 20 mM | |
10 | Sodium chloride | natural abundance | 100 mM | |
11 | DTT | natural abundance | 10 mM | |
12 | NaN3 | natural abundance | 0.01 % | |
13 | H2O | natural abundance | 90 % | |
14 | D2O | natural abundance | 10 % |