Structural Mechanism for Bax Inhibition by Cytomegalovirus Protein vMIA
MDGSGEQPRG GGPTSSEQIM KTGALLLQGF IQDRAGRMGG EAPELALDPV PQDASTKKLS ECLKRIGDEL DSNMELQRMI AAVDTDSPRE VFFRVAADMF SDGNFNWGRV VALFYFASKL VLKALCTKVP ELIRTIMGWT LDFLRERLLG WIQDQGGWDG LLSYFGTPTW QTVTIFVAGV LTASLTIWKK MG
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 7.0 % (176 of 2507) | 8.7 % (113 of 1305) | 4.9 % (48 of 976) | 6.6 % (15 of 226) |
Backbone | 7.1 % (90 of 1262) | 10.3 % (45 of 439) | 4.9 % (30 of 618) | 7.3 % (15 of 205) |
Sidechain | 7.0 % (101 of 1437) | 7.9 % (68 of 866) | 6.0 % (33 of 550) | 0.0 % (0 of 21) |
Aromatic | 1.9 % (4 of 214) | 1.9 % (2 of 107) | 2.0 % (2 of 100) | 0.0 % (0 of 7) |
Methyl | 9.2 % (22 of 240) | 8.3 % (10 of 120) | 10.0 % (12 of 120) |
1. entity 1
MDGSGEQPRG GGPTSSEQIM KTGALLLQGF IQDRAGRMGG EAPELALDPV PQDASTKKLS ECLKRIGDEL DSNMELQRMI AAVDTDSPRE VFFRVAADMF SDGNFNWGRV VALFYFASKL VLKALCTKVP ELIRTIMGWT LDFLRERLLG WIQDQGGWDG LLSYFGTPTW QTVTIFVAGV LTASLTIWKK MG2. entity 2
CEALKKALRR HRFLWQRRQR ASolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity_1 | natural abundance | 0.1 mM | |
2 | entity_2 | [U-100% 13C; U-100% 15N] | 0.1 mM | |
3 | potassium phosphate | natural abundance | 20 mM | |
4 | D2O | [U-100% 2H] | 10 % | |
5 | H2O | natural abundance | 90 % | |
6 | DTT | natural abundance | 0 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | entity_1 | [U-100% 15N] | 0.1 mM | |
8 | entity_2 | natural abundance | 0.1 mM | |
9 | potassium phosphate | natural abundance | 20 mM | |
10 | D2O | [U-100% 2H] | 10 % | |
11 | H2O | natural abundance | 90 % | |
12 | DTT | natural abundance | 0 mM |