The chemical shifts and T1, T2, and 1H-15N NOE data for apo-IscU(E111A)
MAYSEKVIDH YENPRNVGSF DNNDENVGSG MVGAPACGDV MKLQIKVNDE GIIEDARFKT YGCGSAIASS SLVTEWVKGK SLDEAQAIKN TDIAEELELP PVKIHCSILA ADAIKAAIAD YKSKREAK
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 33.2 % (472 of 1420) | 17.4 % (128 of 735) | 47.5 % (262 of 551) | 61.2 % (82 of 134) |
Backbone | 55.1 % (419 of 760) | 34.5 % (90 of 261) | 66.1 % (248 of 375) | 65.3 % (81 of 124) |
Sidechain | 17.2 % (134 of 779) | 8.0 % (38 of 474) | 32.2 % (95 of 295) | 10.0 % (1 of 10) |
Aromatic | 2.8 % (2 of 72) | 2.8 % (1 of 36) | 0.0 % (0 of 35) | 100.0 % (1 of 1) |
Methyl | 16.9 % (24 of 142) | 8.5 % (6 of 71) | 25.4 % (18 of 71) |
1. IscU(E111A)
MAYSEKVIDH YENPRNVGSF DNNDENVGSG MVGAPACGDV MKLQIKVNDE GIIEDARFKT YGCGSAIASS SLVTEWVKGK SLDEAQAIKN TDIAEELELP PVKIHCSILA ADAIKAAIAD YKSKREAKSolvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | IscU(E111A) | [U-13C; U-15N] | 1.5 ~ 2.0 mM | |
2 | TRIS | natural abundance | 20 mM | |
3 | DTT | natural abundance | 5 mM | |
4 | EDTA | natural abundance | 0.5 mM | |
5 | sodium chloride | natural abundance | 150 mM | |
6 | DSS | natural abundance | 0.7 mM | |
7 | D2O | [U-99% 2H] | 7 % | |
8 | sodium azide | natural abundance | 0.02 % | |
9 | H2O | [U-99% 2H] | 93 % |