The chemical shifts and T1, T2, and 1H-15N NOE data for apo-IscU(N90A)
MAYSEKVIDH YENPRNVGSF DNNDENVGSG MVGAPACGDV MKLQIKVNDE GIIEDARFKT YGCGSAIASS SLVTEWVKGK SLDEAQAIKA TDIAEELELP PVKIHCSILA EDAIKAAIAD YKSKREAK
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 37.0 % (525 of 1420) | 20.3 % (149 of 735) | 51.4 % (284 of 552) | 69.2 % (92 of 133) |
Backbone | 60.8 % (462 of 760) | 39.5 % (103 of 261) | 72.0 % (270 of 375) | 71.8 % (89 of 124) |
Sidechain | 19.3 % (150 of 779) | 9.7 % (46 of 474) | 34.1 % (101 of 296) | 33.3 % (3 of 9) |
Aromatic | 4.2 % (3 of 72) | 5.6 % (2 of 36) | 0.0 % (0 of 35) | 100.0 % (1 of 1) |
Methyl | 21.8 % (31 of 142) | 16.9 % (12 of 71) | 26.8 % (19 of 71) |
1. IscU(N90A)
MAYSEKVIDH YENPRNVGSF DNNDENVGSG MVGAPACGDV MKLQIKVNDE GIIEDARFKT YGCGSAIASS SLVTEWVKGK SLDEAQAIKA TDIAEELELP PVKIHCSILA EDAIKAAIAD YKSKREAKSolvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | IscU(N90A) | [U-13C; U-15N] | 1.5 ~ 2.0 mM | |
2 | TRIS | natural abundance | 20 mM | |
3 | DTT | natural abundance | 5 mM | |
4 | EDTA | natural abundance | 0.5 mM | |
5 | sodium chloride | natural abundance | 150 mM | |
6 | DSS | natural abundance | 0.7 mM | |
7 | D2O | [U-99% 2H] | 7 % | |
8 | sodium azide | natural abundance | 0.02 % | |
9 | H2O | [U-99% 2H] | 93 % |