NMR structure of the second PHD finger of AIRE (AIRE-PHD2)
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 88.0 % (587 of 667) | 95.3 % (327 of 343) | 76.1 % (197 of 259) | 96.9 % (63 of 65) |
Backbone | 83.2 % (321 of 386) | 97.1 % (133 of 137) | 67.6 % (127 of 188) | 100.0 % (61 of 61) |
Sidechain | 95.5 % (322 of 337) | 94.2 % (194 of 206) | 99.2 % (126 of 127) | 50.0 % (2 of 4) |
Aromatic | 100.0 % (44 of 44) | 100.0 % (22 of 22) | 100.0 % (21 of 21) | 100.0 % (1 of 1) |
Methyl | 100.0 % (54 of 54) | 100.0 % (27 of 27) | 100.0 % (27 of 27) |
1. AIRE-PHD2
GAMEGQQNLA PGARCGVCGD GTDVLRCTHC AAAFHWRCHF PAGTSRPGTG LRCRSCSGDV TPAPVESolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.5, Details 50mM sodium phosphate pH6.5, 0.15M NaCl, 5mM DTT, 50 M ZnCl2, 0.02% NaN3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | AIRE-PHD2 | [U-100% 13C; U-100% 15N] | 0.4 ~ 0.8 mM | |
2 | ZINC ION | natural abundance | 50 uM | |
3 | DTT | natural abundance | 5 mM | |
4 | sodium phosphate | natural abundance | 50 mM | |
5 | sodium chloride | natural abundance | 0.15 M | |
6 | sodium azide | natural abundance | 0.02 % | |
7 | D2O | natural abundance | 10 % | |
8 | H2O | natural abundance | 90 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 295 K, pH 6.5, Details 50mM sodium phosphate pH6.5, 0.15M NaCl, 5mM DTT, 50 M ZnCl2, 0.02% NaN3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | AIRE-PHD2 | [U-100% 13C; U-100% 15N] | 0.4 ~ 0.8 mM | |
10 | DTT | natural abundance | 5 mM | |
11 | ZINC ION | natural abundance | 50 uM | |
12 | sodium phosphate | natural abundance | 50 mM | |
13 | sodium chloride | natural abundance | 0.15 M | |
14 | sodium azide | natural abundance | 0.02 % | |
15 | D2O | natural abundance | 100 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.5, Details 50mM sodium phosphate pH6.5, 0.15M NaCl, 5mM DTT, 50 M ZnCl2, 0.02% NaN3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
16 | AIRE-PHD2 | [U-100% 15N] | 0.4 ~ 0.8 mM | |
17 | DTT | natural abundance | 5 mM | |
18 | ZINC ION | natural abundance | 50 uM | |
19 | sodium phosphate | natural abundance | 50 mM | |
20 | sodium chloride | natural abundance | 0.15 M | |
21 | sodium azide | natural abundance | 0.02 % | |
22 | D2O | natural abundance | 10 % | |
23 | H2O | natural abundance | 90 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.5, Details 50mM sodium phosphate pH6.5, 0.15M NaCl, 5mM DTT, 50 M ZnCl2, 0.02% NaN3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
24 | AIRE-PHD2 | natural abundance | 0.4 ~ 0.8 mM | |
25 | DTT | natural abundance | 5 mM | |
26 | ZINC ION | natural abundance | 50 uM | |
27 | sodium phosphate | natural abundance | 50 mM | |
28 | sodium chloride | natural abundance | 0.15 M | |
29 | sodium azide | natural abundance | 0.02 % | |
30 | D2O | natural abundance | 10 % | |
31 | H2O | natural abundance | 90 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 295 K, pH 6.5, Details 50mM sodium phosphate pH6.5, 0.15M NaCl, 5mM DTT, 50 M ZnCl2, 0.02% NaN3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
32 | AIRE-PHD2 | natural abundance | 0.4 ~ 0.8 mM | |
33 | DTT | natural abundance | 5 mM | |
34 | ZINC ION | natural abundance | 50 uM | |
35 | sodium phosphate | natural abundance | 50 mM | |
36 | sodium chloride | natural abundance | 0.15 M | |
37 | sodium azide | natural abundance | 0.02 % | |
38 | D2O | natural abundance | 100 % |