BMRB: 18386

Characterization of the chromoshadow domain-mediated binding of heterochromatin protein 1 alpha (HP1 alpha) to histone H3: Backbone 1H, 15N chemical shift assignments for HP1 CSDalpha(109-185)

Authors

Richart, A.N., Brunner, C.I.W., Stott, K., Murzina, N.V., Thomas, J.O.

Assembly

HP1 CSDalpha(109-185)

Entity

1. HP1 CSDalpha(109-185) (polymer, Thiol state: all free), 191 monomers, 22224.71 Da Detail

MGKKTKRTAD SSSSEDEEEY VVEKVLDRRV VKGQVEYLLK WKGFSEEHNT WEPEKNLDCP ELISEFMKKY KKMKEGENNK PREKSESNKR KSNFSNSADD IKSKKKREQS NDIARGFERG LEPEKIIGAT DSCGDLMFLM KWKDTDEADL VLAKEANVKC PQIVIAFYEE RLTWHAYPED AENKEKETAK S

Formula weight

22224.71 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 37.2 %, Completeness: 11.6 %, Completeness (bb): 25.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	11.6 % (166 of 1428)	7.5 % (92 of 1226)	36.6 % (74 of 202)
Backbone	25.5 % (145 of 569)	19.5 % (75 of 384)	37.8 % (70 of 185)
Sidechain	2.4 % (21 of 859)	2.0 % (17 of 842)	23.5 % (4 of 17)
Aromatic	4.9 % (4 of 82)	2.6 % (2 of 78)	50.0 % (2 of 4)
Methyl	1.4 % (1 of 72)	1.4 % (1 of 72)

1. HP1 CSDalpha(109-185)

MGKKTKRTAD SSSSEDEEEY VVEKVLDRRV VKGQVEYLLK WKGFSEEHNT WEPEKNLDCP ELISEFMKKY KKMKEGENNK PREKSESNKR KSNFSNSADD IKSKKKREQS NDIARGFERG LEPEKIIGAT DSCGDLMFLM KWKDTDEADL VLAKEANVKC PQIVIAFYEE RLTWHAYPED AENKEKETAK S

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 8.0

#	Name	Isotope labeling	Type	Concentration
1	HP1_CSDalpha(109-185)	[U-95% 15N]		0.5 mM
2	sodium phosphate	natural abundance		10 mM
3	EDTA	natural abundance		1 mM
4	DTT	natural abundance		1 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

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Release date

2012-05-20

Citation

Characterization of chromoshadow domain-mediated binding of heterochromatin protein 1α (HP1α) to histone H3
Richart, A.N., Brunner, C.I.W., Stott, K., Murzina, N.V., Thomas, J.O.
J. Biol. Chem. (2012), 287, 18730-18737, PubMed 22493481 , DOI 10.1074/jbc.M111.337204 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. HP1 CSDalpha(109-185), : 4 : 26 entities Detail

Interaction partners 1. HP1 CSDalpha(109-185), : 72 interactors Detail

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail