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High Definition Solution Structure of PED/PEA-15 Death Effector Domain
Authors
Twomey, E.C., Wei, Y.
Assembly
phosphoprotein enriched in astrocytes 15A
Entity
1. phosphoprotein enriched in astrocytes 15A (polymer), 130 monomers, 15039.91 Da Detail

MAEYGTLLQD LTNNITLEDL EQLKSACKED IPSEKSEEIT TGSAWFSFLE SHNKLDKDNL SYIEHIFEIS RRPDLLTMVV DYRTRVLKIS EEDELDTKLT RIPSAKKYKD IIRQPSEEEI IKLAPPPKKA


Formula weight
15039.91 Da
Source organism
Mus musculus
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 99.2 %, Completeness: 84.1 %, Completeness (bb): 83.2 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All84.1 % (1318 of 1567)84.6 % (697 of 824)80.7 % (494 of 612)96.9 % (127 of 131)
Backbone83.2 % (637 of 766)95.3 % (243 of 255)70.9 % (275 of 388)96.7 % (119 of 123)
Sidechain85.3 % (792 of 929)78.6 % (447 of 569)95.7 % (337 of 352)100.0 % (8 of 8)
Aromatic100.0 % (82 of 82)100.0 % (41 of 41)100.0 % (40 of 40)100.0 % (1 of 1)
Methyl96.0 % (144 of 150)96.0 % (72 of 75)96.0 % (72 of 75)

1. pea-15

MAEYGTLLQD LTNNITLEDL EQLKSACKED IPSEKSEEIT TGSAWFSFLE SHNKLDKDNL SYIEHIFEIS RRPDLLTMVV DYRTRVLKIS EEDELDTKLT RIPSAKKYKD IIRQPSEEEI IKLAPPPKKA

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 7


#NameIsotope labelingTypeConcentration
1phosphoprotein enriched in astrocytes 15A[U-100% 13C; U-100% 15N]1 mM
2H2Onatural abundance93 %
3D2Onatural abundance7 %

LACS Plot; CA
Referencing offset: -0.08 ppm, Outliers: 4 Detail
LACS Plot; CB
Referencing offset: -0.08 ppm, Outliers: 4 Detail
LACS Plot; HA
Referencing offset: 0.07 ppm, Outliers: 3 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2LS7, Strand ID: A Detail


Release date
2012-08-05
Citation
High-definition NMR structure of PED/PEA-15 death effector domain reveals details of key polar side chain interactions
Twomey, E.C., Wei, Y.
Biochem. Biophys. Res. Commun. (2012), 424, 141-146, PubMed 22732408 , DOI 10.1016/j.bbrc.2012.06.091 ,
Related entities 1. phosphoprotein enriched in astrocytes 15A, : 6 : 2 : 17 entities Detail
Interaction partners 1. phosphoprotein enriched in astrocytes 15A, : 13 interactors Detail
Experiments performed 10 experiments Detail
NMR combined restraints 7 contents Detail
Keywords death domain superfamily, death effector domain, six helix bundle