1H,15N and 13C backbone and side chain chemical shifts of the ubiquitin homology domain of mouse BAG-1
MAKTEEMVQT EEMETPRLSV IVTHSNERYD LLVTPQQGNS EPVVQDLAQL VEEATGVPLP FQKLIFKGKS LKEMETPLSA LGMQNGCRVM LIGEKSN
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 90.1 % (1017 of 1129) | 89.9 % (532 of 592) | 89.4 % (389 of 435) | 94.1 % (96 of 102) |
Backbone | 94.0 % (536 of 570) | 93.3 % (181 of 194) | 94.7 % (270 of 285) | 93.4 % (85 of 91) |
Sidechain | 87.7 % (570 of 650) | 88.2 % (351 of 398) | 86.3 % (208 of 241) | 100.0 % (11 of 11) |
Aromatic | 28.1 % (9 of 32) | 56.3 % (9 of 16) | 0.0 % (0 of 16) | |
Methyl | 93.0 % (106 of 114) | 94.7 % (54 of 57) | 91.2 % (52 of 57) |
1. ubiquitin homology domain of mouse BAG-1
MAKTEEMVQT EEMETPRLSV IVTHSNERYD LLVTPQQGNS EPVVQDLAQL VEEATGVPLP FQKLIFKGKS LKEMETPLSA LGMQNGCRVM LIGEKSNSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | ubiquitin homology domain of mouse BAG-1 | [U-100% 13C; U-100% 15N] | 1.0 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 100 mM | |
4 | DTT | natural abundance | 5 mM | |
5 | H2O | natural abundance | 90 % | |
6 | D2O | natural abundance | 10 % |