Solution structure of gpFI C-terminal domain
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 85.2 % (562 of 660) | 94.6 % (314 of 332) | 70.3 % (189 of 269) | 100.0 % (59 of 59) |
Backbone | 83.1 % (299 of 360) | 98.4 % (120 of 122) | 67.2 % (121 of 180) | 100.0 % (58 of 58) |
Sidechain | 89.1 % (319 of 358) | 92.4 % (194 of 210) | 84.4 % (124 of 147) | 100.0 % (1 of 1) |
Aromatic | 32.1 % (9 of 28) | 64.3 % (9 of 14) | 0.0 % (0 of 14) | |
Methyl | 91.0 % (91 of 100) | 92.0 % (46 of 50) | 90.0 % (45 of 50) |
1. polypeptide
PDTVILDTSE LVTVVALVKL HTDALHATRD EPVAFVLPGT AFRVSAGVAA EMTERGLARM QSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | gpFI | [U-99% 13C; U-99% 15N] | 1 mM | |
2 | sodium phosphate | natural abundance | 25 mM | |
3 | sodium chloride | natural abundance | 250 mM | |
4 | H2O | natural abundance | 90 mM | |
5 | D2O | natural abundance | 10 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | gpFI | [U-100% 13C; U-100% 15N] | 1 mM | |
7 | sodium phosphate | natural abundance | 25 mM | |
8 | potassium chloride | natural abundance | 250 mM | |
9 | D2O | natural abundance | 100 % |