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BMRB: 18467

2LTC

FAS1-4, R555W

Authors

Underhaug, J., Nielsen, N., Runager, K.

Assembly

FAS1-4 R555W

Entity

1. FAS1-4 R555W (polymer, Thiol state: not present), 135 monomers, 14469.53 Da Detail

AGMGTVMDVL KGDNRFSMLV AAIQSAGLTE TLNREGVYTV FAPTNEAFRA LPPREWSRLL GDAKELANIL KYHIGDEILV SGGIGALVRL KSLQGDKLEV SLKNNVVSVN KEPVAEPDIM ATNGVVHVIT NVLQA

Formula weight

14469.53 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 86.8 %, Completeness (bb): 95.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.8 % (1318 of 1519)	84.9 % (662 of 780)	87.9 % (524 of 596)	92.3 % (132 of 143)
Backbone	95.5 % (764 of 800)	95.3 % (264 of 277)	95.4 % (375 of 393)	96.2 % (125 of 130)
Sidechain	80.0 % (674 of 842)	79.1 % (398 of 503)	82.5 % (269 of 326)	53.8 % (7 of 13)
Aromatic	81.8 % (54 of 66)	87.9 % (29 of 33)	75.0 % (24 of 32)	100.0 % (1 of 1)
Methyl	95.4 % (187 of 196)	99.0 % (97 of 98)	91.8 % (90 of 98)

1. FAS1-4 R555W

AGMGTVMDVL KGDNRFSMLV AAIQSAGLTE TLNREGVYTV FAPTNEAFRA LPPREWSRLL GDAKELANIL KYHIGDEILV SGGIGALVRL KSLQGDKLEV SLKNNVVSVN KEPVAEPDIM ATNGVVHVIT NVLQA

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	FAS1-4, R555W	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	DSS	natural abundance	0.5 mM
5	sodium azide	natural abundance	0.5 %
6	arginine	natural abundance	50 mM
7	glutamate	natural abundance	50 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.48 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2LTC, Strand ID: A Detail

Release date

2013-08-14

Citation

Mutation in transforming growth factor beta induced protein associated with granular corneal dystrophy type 1 reduces the proteolytic susceptibility through local structural stabilization
Underhaug, J., Kolds, H., Runager, K., Nielsen, J., Srensen, C.S., Kristensen, T., Otzen, D.E., Karring, H., Malmendal, A., Schitt, B., Enghild, J.J., Nielsen, N.
Biochim. Biophys. Acta (2013), 1834, 2812-2822, PubMed 24129074 , DOI 10.1016/j.bbapap.2013.10.008 , Abstract

Related entities 1. FAS1-4 R555W, : 1 : 8 : 130 entities Detail

Interaction partners 1. FAS1-4 R555W, : 3 interactors Detail

More details for 3 interactors identified by 2 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC