BMRB: 18477

NMR dynamics in the C-terminal globular domain of oligosaccharyltransferase

Authors

Nyirenda, J., Matsumoto, S., Takashi, S., Kohda, D.

Assembly

A.fAglB S2

Entity

1. A.fAglB S2 (polymer, Thiol state: not present), 180 monomers, 21170.14 Da Detail

MAHHHHHHSA ALEVLFQGPE MTMDWKEALN WMKENLEAQD YLKAYEKPDY AVLSWWDYGN WILYVAKKAV VCNNFQAGAD DAAKFFTAQS EEEAMKIVEK RKVRYVVTVE ELTVKPETNK TKFIPIMQIA GYSPEYMKNK EIIDFFNKTM LYKLHVENAT NLTHFRLLKN FGTVKIFEVK

Formula weight

21170.14 Da

Source organism

Archaeoglobus fulgidus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation, order_parameters

Chem. Shift Complete

Sequence coverage: 86.1 %, Completeness: 25.6 %, Completeness (bb): 58.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	25.6 % (347 of 1353)	16.4 % (190 of 1157)	80.1 % (157 of 196)
Backbone	58.9 % (315 of 535)	44.4 % (160 of 360)	88.6 % (155 of 175)
Sidechain	3.9 % (32 of 818)	3.8 % (30 of 797)	9.5 % (2 of 21)
Aromatic	0.0 % (0 of 137)	0.0 % (0 of 132)	0.0 % (0 of 5)
Methyl	4.1 % (4 of 97)	4.1 % (4 of 97)

1. AfAglB-S2

MAHHHHHHSA ALEVLFQGPE MTMDWKEALN WMKENLEAQD YLKAYEKPDY AVLSWWDYGN WILYVAKKAV VCNNFQAGAD DAAKFFTAQS EEEAMKIVEK RKVRYVVTVE ELTVKPETNK TKFIPIMQIA GYSPEYMKNK EIIDFFNKTM LYKLHVENAT NLTHFRLLKN FGTVKIFEVK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0, Details Added DTT to suppress oligomerisation

#	Name	Isotope labeling	Type	Concentration
1	AfAglB-S2	[U-99% 13C; U-99% 15N]		1.0 mM
2	AfAglB-S2	[U-99% 15N]		1.0 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0, Details For relaxation studies

#	Name	Isotope labeling	Type	Concentration
5	AfAglB-S2	[U-99% 15N]		0.5-1.0 mM
6	H2O	natural abundance		90 %
7	D2O	natural abundance		10 %

Hide sample codition

Heteronucl. T₁

264 T₁ values in 2 lists
Coherence Sz, Field strength (¹H) 600 MHz, 700 MHz, Pressure 1 atm, Temperature 308 K, pH 7.0 Detail

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Pressure 1 atm, Temperature 308 K, pH 7.0

Experiment name 2D 1H-15N HSQC

Heteronucl. T₂

264 T₂ values in 2 lists
Coherence S(+,-), Field strength (¹H) 600 MHz, 700 MHz, Pressure 1 atm, Temperature 308 K, pH 7.0 Detail

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Pressure 1 atm, Temperature 308 K, pH 7.0

Experiment name 2D 1H-15N HSQC

Heteronucl. NOE

264 NOE values in 2 lists
Value type relative intensities, Field strength (¹H) 600 MHz, 700 MHz, Pressure 1 atm, Temperature 308 K, pH 7.0 Detail

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Pressure 1 atm, Temperature 308 K, pH 7.0

Experiment name 1H-15N heteronuclear noe

Heteronucl. T₁/T₂

264 T₁/T₂ values in 2 lists
Field strength (¹H) 600 MHz, 700 MHz, Pressure 1 atm, Temperature 308 K, pH 7.0 Detail

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Pressure 1 atm, Temperature 308 K, pH 7.0

Order parameters

130 S² values in 1 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 308 K, pH 7.0 Detail

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Pressure 1 atm, Temperature 308 K, pH 7.0

Experiment name 2D 1H-15N HSQC

List #1 order_parameter_list_1, Field strength (¹H) 600 MHz

Release date

2012-11-25

Citation

Crystallographic and NMR evidence for flexibility in oligosaccharyltransferases and its catalytic significance
Nyirenda, J., Matsumoto, S., Saitoh, T., Maita, N., Noda, N.N., Inagaki, F., Kohda, D.
Structure (2013), 21, 32-41, PubMed 23177926 , DOI 10.1016/j.str.2012.10.011 , Abstract

Related entities 1. A.fAglB S2, : 1 : 24 entities Detail

Experiments performed 9 experiments Detail

Chemical shift validation 3 contents Detail