NMR structure of major ampullate spidroin 1 N-terminal domain at pH 5.5
GSGNSHTTPW TNPGLAENFM NSFMQGLSSM PGFTASQLDD MSTIAQSMVQ SIQSLAAQGR TSPNKLQALN MAFASSMAEI AASEEGGGSL STKTSSIASA MSNAFLQTTG VVNQPFINEI TQLVSMFAQA GMNDVSA
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 96.7 % (1416 of 1465) | 97.5 % (730 of 749) | 95.2 % (535 of 562) | 98.1 % (151 of 154) |
Backbone | 95.4 % (775 of 812) | 97.1 % (272 of 280) | 93.5 % (374 of 400) | 97.7 % (129 of 132) |
Sidechain | 98.3 % (766 of 779) | 97.7 % (458 of 469) | 99.3 % (286 of 288) | 100.0 % (22 of 22) |
Aromatic | 100.0 % (86 of 86) | 100.0 % (43 of 43) | 100.0 % (42 of 42) | 100.0 % (1 of 1) |
Methyl | 98.5 % (134 of 136) | 97.1 % (66 of 68) | 100.0 % (68 of 68) |
1. Major ampullate spidroin 1
GSGNSHTTPW TNPGLAENFM NSFMQGLSSM PGFTASQLDD MSTIAQSMVQ SIQSLAAQGR TSPNKLQALN MAFASSMAEI AASEEGGGSL STKTSSIASA MSNAFLQTTG VVNQPFINEI TQLVSMFAQA GMNDVSASolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Major ampullate spidroin 1 | [U-99% 13C; U-99% 15N] | 1.5 mM | |
2 | D2O | [U-100% 2H] | 5 % | |
3 | sodium azide | natural abundance | 0.03 % | |
4 | sodium acetate | natural abundance | 20 mM | |
5 | sodium chloride | natural abundance | 20 mM | |
6 | H2O | natural abundance | 95 % |