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BMRB: 18492

2LTP

Solution structure of the SANT2 domain of the human nuclear receptor corepressor 2 (NCoR2), Northeast Structural Genomics Consortium (NESG) target ID HR4636E.

Authors

Montecchio, M., Lemak, A., Yee, A., Xu, C., Garcia, M., Houliston, S., Bellanda, M., Min, J., Arrowsmith, C., Montelione, G.

Assembly

SANT2 domain of NCoR2

Entity

1. SANT2 domain of NCoR2 (polymer, Thiol state: all free), 89 monomers, 10748.15 Da Detail

MHHHHHHSSG RENLYFQGWT EEEMGTAKKG LLEHGRNWSA IARMVGSKTV SQCKNFYFNY KKRQNLDEIL QQHKLKMEKE RNARRKKKK

Formula weight

10748.15 Da

Source organism

Exptl. method

Refine. method

restrained molecular dynamics

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 78.7 %, Completeness: 70.1 %, Completeness (bb): 70.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	70.1 % (786 of 1121)	70.5 % (423 of 600)	69.2 % (290 of 419)	71.6 % (73 of 102)
Backbone	70.4 % (376 of 534)	71.7 % (132 of 184)	69.3 % (181 of 261)	70.8 % (63 of 89)
Sidechain	70.0 % (469 of 670)	70.0 % (291 of 416)	69.7 % (168 of 241)	76.9 % (10 of 13)
Aromatic	57.3 % (63 of 110)	60.0 % (33 of 55)	52.8 % (28 of 53)	100.0 % (2 of 2)
Methyl	92.6 % (50 of 54)	92.6 % (25 of 27)	92.6 % (25 of 27)

1. entity 1

MHHHHHHSSG RENLYFQGWT EEEMGTAKKG LLEHGRNWSA IARMVGSKTV SQCKNFYFNY KKRQNLDEIL QQHKLKMEKE RNARRKKKK

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	SANT2	[U-13C; U-15N]		1 mM
2	sodium phosphate	natural abundance		25 mM
3	NaCl	natural abundance		200 mM
4	ZnSO4	natural abundance		0.01 mM
5	DTT	natural abundance		10 mM
6	benzamidine	natural abundance		1 mM
7	NaN3	natural abundance		0.01 %
8	H2O	natural abundance		95 %
9	D2O	natural abundance		5 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2LTP, Strand ID: A Detail

Release date

2012-06-17

Citation

Solution structure of the SANT2 domain of the human nuclear receptor corepressor 2 (NCoR2)
Montecchio, M., Lemak, A., Yee, A., Xu, C., Garcia, M., Houliston, S., Bellanda, M., Min, J., Arrowsmith, C.
Not known

Related entities 1. SANT2 domain of NCoR2, : 1 : 14 entities Detail

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	SANT2	[U-13C; U-15N]		1 mM
2	sodium phosphate	natural abundance		25 mM
3	NaCl	natural abundance		200 mM
4	ZnSO4	natural abundance		0.01 mM
5	DTT	natural abundance		10 mM
6	benzamidine	natural abundance		1 mM
7	NaN3	natural abundance		0.01 %
8	H2O	natural abundance		95 %
9	D2O	natural abundance		5 %

2 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	SANT2	[U-13C; U-15N]		1 mM
2	sodium phosphate	natural abundance		25 mM
3	NaCl	natural abundance		200 mM
4	ZnSO4	natural abundance		0.01 mM
5	DTT	natural abundance		10 mM
6	benzamidine	natural abundance		1 mM
7	NaN3	natural abundance		0.01 %
8	H2O	natural abundance		95 %
9	D2O	natural abundance		5 %

3 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	SANT2	[U-13C; U-15N]		1 mM
2	sodium phosphate	natural abundance		25 mM
3	NaCl	natural abundance		200 mM
4	ZnSO4	natural abundance		0.01 mM
5	DTT	natural abundance		10 mM
6	benzamidine	natural abundance		1 mM
7	NaN3	natural abundance		0.01 %
8	H2O	natural abundance		95 %
9	D2O	natural abundance		5 %

4 3D HNCA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	SANT2	[U-13C; U-15N]		1 mM
2	sodium phosphate	natural abundance		25 mM
3	NaCl	natural abundance		200 mM
4	ZnSO4	natural abundance		0.01 mM
5	DTT	natural abundance		10 mM
6	benzamidine	natural abundance		1 mM
7	NaN3	natural abundance		0.01 %
8	H2O	natural abundance		95 %
9	D2O	natural abundance		5 %

5 3D HBHA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	SANT2	[U-13C; U-15N]		1 mM
2	sodium phosphate	natural abundance		25 mM
3	NaCl	natural abundance		200 mM
4	ZnSO4	natural abundance		0.01 mM
5	DTT	natural abundance		10 mM
6	benzamidine	natural abundance		1 mM
7	NaN3	natural abundance		0.01 %
8	H2O	natural abundance		95 %
9	D2O	natural abundance		5 %

6 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	SANT2	[U-13C; U-15N]		1 mM
2	sodium phosphate	natural abundance		25 mM
3	NaCl	natural abundance		200 mM
4	ZnSO4	natural abundance		0.01 mM
5	DTT	natural abundance		10 mM
6	benzamidine	natural abundance		1 mM
7	NaN3	natural abundance		0.01 %
8	H2O	natural abundance		95 %
9	D2O	natural abundance		5 %

7 2D 1H-13C HSQC aliphatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	SANT2	[U-13C; U-15N]		1 mM
2	sodium phosphate	natural abundance		25 mM
3	NaCl	natural abundance		200 mM
4	ZnSO4	natural abundance		0.01 mM
5	DTT	natural abundance		10 mM
6	benzamidine	natural abundance		1 mM
7	NaN3	natural abundance		0.01 %
8	H2O	natural abundance		95 %
9	D2O	natural abundance		5 %

8 2D 1H-13C HSQC aromatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	SANT2	[U-13C; U-15N]		1 mM
2	sodium phosphate	natural abundance		25 mM
3	NaCl	natural abundance		200 mM
4	ZnSO4	natural abundance		0.01 mM
5	DTT	natural abundance		10 mM
6	benzamidine	natural abundance		1 mM
7	NaN3	natural abundance		0.01 %
8	H2O	natural abundance		95 %
9	D2O	natural abundance		5 %

9 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	SANT2	[U-13C; U-15N]		1 mM
2	sodium phosphate	natural abundance		25 mM
3	NaCl	natural abundance		200 mM
4	ZnSO4	natural abundance		0.01 mM
5	DTT	natural abundance		10 mM
6	benzamidine	natural abundance		1 mM
7	NaN3	natural abundance		0.01 %
8	H2O	natural abundance		95 %
9	D2O	natural abundance		5 %

10 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	SANT2	[U-13C; U-15N]		1 mM
2	sodium phosphate	natural abundance		25 mM
3	NaCl	natural abundance		200 mM
4	ZnSO4	natural abundance		0.01 mM
5	DTT	natural abundance		10 mM
6	benzamidine	natural abundance		1 mM
7	NaN3	natural abundance		0.01 %
8	H2O	natural abundance		95 %
9	D2O	natural abundance		5 %

11 3D H(C)CH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	SANT2	[U-13C; U-15N]		1 mM
2	sodium phosphate	natural abundance		25 mM
3	NaCl	natural abundance		200 mM
4	ZnSO4	natural abundance		0.01 mM
5	DTT	natural abundance		10 mM
6	benzamidine	natural abundance		1 mM
7	NaN3	natural abundance		0.01 %
8	H2O	natural abundance		95 %
9	D2O	natural abundance		5 %

12 3D (H)CCH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	SANT2	[U-13C; U-15N]		1 mM
2	sodium phosphate	natural abundance		25 mM
3	NaCl	natural abundance		200 mM
4	ZnSO4	natural abundance		0.01 mM
5	DTT	natural abundance		10 mM
6	benzamidine	natural abundance		1 mM
7	NaN3	natural abundance		0.01 %
8	H2O	natural abundance		95 %
9	D2O	natural abundance		5 %

nullKeywords NCoR2, NESG, Northeast Structural Genomics Consortium, Nuclear receptor corepressor 2, SANT, SGC, Structural Genomics Consortium