Solution Structure of autoinhibitory domain of human AMP-activated protein kinase catalytic subunit
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 86.1 % (597 of 693) | 84.7 % (304 of 359) | 86.5 % (231 of 267) | 92.5 % (62 of 67) |
Backbone | 97.6 % (359 of 368) | 97.6 % (121 of 124) | 97.3 % (179 of 184) | 98.3 % (59 of 60) |
Sidechain | 77.4 % (298 of 385) | 77.9 % (183 of 235) | 78.3 % (112 of 143) | 42.9 % (3 of 7) |
Aromatic | 9.5 % (4 of 42) | 19.0 % (4 of 21) | 0.0 % (0 of 21) | |
Methyl | 96.7 % (58 of 60) | 96.7 % (29 of 30) | 96.7 % (29 of 30) |
1. entity
GPHMSYDANV IDDEAVKEVC EKFECTESEV MNSLYSGDPQ DQLAVAYHLI IDNRRIMNQA SESolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 15N/13C labeled AID protein
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | AID protein | [U-100% 13C; U-100% 15N] | 0.2 mM | |
2 | sodium phosphate | natural abundance | 50 mM | |
3 | D2O | [U-100% 2H] | 10 % | |
4 | H2O | natural abundance | 90 % | |
5 | EDTA | natural abundance | 1 mM |