Solution NMR structure of C2H2-type Zinc-fingers 4 and 5 from human Insulinoma-associated protein 1 (fragment 424-497), Northeast Structural Genomics Consortium Target HR7614B.
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | metal coordination | sing | 1:CYS31:SG | 2:ZN1:ZN |
2 | metal coordination | sing | 1:CYS34:SG | 2:ZN1:ZN |
3 | metal coordination | sing | 1:HIS47:NE2 | 2:ZN1:ZN |
4 | metal coordination | sing | 1:HIS52:NE2 | 2:ZN1:ZN |
5 | metal coordination | sing | 1:CYS59:SG | 2:ZN1:ZN |
6 | metal coordination | sing | 1:CYS62:SG | 2:ZN1:ZN |
7 | metal coordination | sing | 1:HIS75:NE2 | 2:ZN1:ZN |
8 | metal coordination | sing | 1:HIS80:NE2 | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 86.5 % (800 of 925) | 87.0 % (416 of 478) | 86.0 % (312 of 363) | 85.7 % (72 of 84) |
Backbone | 86.8 % (434 of 500) | 87.4 % (152 of 174) | 87.0 % (214 of 246) | 85.0 % (68 of 80) |
Sidechain | 86.6 % (434 of 501) | 86.8 % (264 of 304) | 86.0 % (166 of 193) | 100.0 % (4 of 4) |
Aromatic | 66.0 % (62 of 94) | 66.0 % (31 of 47) | 66.0 % (31 of 47) | |
Methyl | 100.0 % (64 of 64) | 100.0 % (32 of 32) | 100.0 % (32 of 32) |
1. HR7614B
MGHHHHHHSH MGDGEGAGVL GLSASAECHL CPVCGESFAS KGAQERHLRL LHAAQVFPCK YCPATFYSSP GLTRHINKCH PSENRSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.69 mM HR7614B.021, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR7614B.021 | [U-100% 13C; U-100% 15N] | 0.69 mM | |
2 | NaCl | natural abundance | 100 mM | |
3 | DTT | natural abundance | 5 mM | |
4 | NaN3 | natural abundance | 0.02 % | |
5 | Tris-HCl pH 7.5 | natural abundance | 10 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.25 mM HR7614B.023, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | HR7614B.023 | [U-5% 13C; U-100% 15N] | 1.25 mM | |
7 | NaCl | natural abundance | 100 mM | |
8 | DTT | natural abundance | 5 mM | |
9 | NaN3 | natural abundance | 0.02 % | |
10 | Tris-HCl pH 7.5 | natural abundance | 10 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.69 mM HR7614B.023
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | HR7614B.023 | [U-5% 13C; U-100% 15N] | 1.25 mM | |
12 | NaCl | natural abundance | 100 mM | |
13 | DTT | natural abundance | 5 mM | |
14 | NaN3 | natural abundance | 0.02 % | |
15 | Tris-HCl pH 7.5 | natural abundance | 10 mM |