The complex between Ca-Calmodulin and skeletal muscle myosin light chain kinase from combination of NMR and aqueous and contrast-matched SAXS data
ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGFI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVTMMTAK
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 27.1 % (332 of 1224) | 17.9 % (186 of 1038) | 78.5 % (146 of 186) |
Backbone | 56.4 % (299 of 530) | 43.0 % (154 of 358) | 84.3 % (145 of 172) |
Sidechain | 4.8 % (33 of 694) | 4.7 % (32 of 680) | 7.1 % (1 of 14) |
Aromatic | 0.0 % (0 of 68) | 0.0 % (0 of 67) | 0.0 % (0 of 1) |
Methyl | 6.0 % (5 of 84) | 6.0 % (5 of 84) |
1. Calmodulin
ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGFI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVTMMTAK2. ssMLCK
KRRWKKNFIA VSAANRFKKI SSSGALSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Calmodulin | [U-13C; U-15N; U-2H] | 0.3 mM | |
2 | ssMLCK | natural abundance | 0.3 mM | |
3 | CaCl2 | natural abundance | 3 mM |
Solvent system 65% w/v sucrose/H2O, Pressure 1 atm, Temperature 273 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | Calmodulin | [U-13C; U-15N; U-2H] | 0.06 ~ 0.25 mM | |
5 | ssMLCK | natural abundance | 0.06 ~ 0.25 mM | |
6 | CaCl2 | natural abundance | 3 mM |