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Found 1 Document (0.712 seconds)

BMRB: 18558

2LV8

Solution NMR Structure de novo designed rossmann 2x2 fold protein, Northeast Structural Genomics Consortium (NESG) Target OR16

Authors: Liu, G., Koga, R., Koga, N., Xiao, R., Pederson, K., Hamilton, K., Ciccosanti, C., Acton, T.B., Everett, J.K., Baker, D., Montelione, G.T.
Assembly: OR16
Entity: 1. OR16 (polymer, Thiol state: not present), 110 monomers, 13050.78 Da Detail
MLLYVLIISN DKKLIEEARK MAEKANLELR TVKTEDELKK YLEEFRKESQ NIKVLILVSN DEELDKAKEL AQKMEIDVRT RKVTSPDEAK RWIKEFSEEG GSLEHHHHHH
Formula weight: 13050.78 Da
Exptl. method: solution NMR
Refine. method: distance geometry, simulated annealing, torsion angle dynamics, molecular dynamics
Data set: RDCs, spectral_peak_list
Protein Blocks Logo: Calculated from 20 models in PDB: 2LV8, Strand ID: A Detail
RDC: 170 RDC values in 2 lists
Field strength (¹H) 599.520 MHz, Pressure 1 atm, Temperature 298 K, pH 6.5 Detail
Pressure 1 atm, Temperature 298 K, pH 6.5
Experiment name 2D 1H-15N HSQC
List #1 RDC_list_1, RDC code DHN, Field strength (¹H) 599.520 MHz, Details alignment medias: peg for Ori 1 and phage for Ori 2. # Orientation Magnitude Rhombicity ORI residue number 1 -7.005 0.392 118 2 10.199 0.418 125
List #2 RDC_list_2, RDC code DHN, Field strength (¹H) 599.520 MHz, Details alignment medias: peg for Ori 1 and phage for Ori 2. # Orientation Magnitude Rhombicity ORI residue number # 2 -12.87 0.278 125
Release date: 2012-07-30
Citation: Principles for designing ideal protein structures
Koga, N., Tatsumi-Koga, R., Liu, G., Xiao, R., Acton, T.B., Montelione, G.T., Baker, D.
Nature (2012), 491, 222-227, PubMed 23135467 , DOI 10.1038/nature11600 , Abstract

Related entities 1. OR16, : 1 : 1 : 80 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz Rutgers

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.073 mM OR16.004, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	OR16.004	[U-100% 13C; U-100% 15N]	1.073 mM
2	Proteinase Inhibitors	natural abundance	1 na
3	NaN3	natural abundance	0.02 %
4	DTT	natural abundance	10 mM
5	CaCL2	natural abundance	5 mM
6	NaCL	natural abundance	200 mM
7	MES pH 6.5	natural abundance	20 mM
8	D2O	natural abundance	10 %
9	DSS	natural abundance	50 uM

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz Rutgers

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.073 mM OR16.004, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	OR16.004	[U-100% 13C; U-100% 15N]	1.073 mM
2	Proteinase Inhibitors	natural abundance	1 na
3	NaN3	natural abundance	0.02 %
4	DTT	natural abundance	10 mM
5	CaCL2	natural abundance	5 mM
6	NaCL	natural abundance	200 mM
7	MES pH 6.5	natural abundance	20 mM
8	D2O	natural abundance	10 %
9	DSS	natural abundance	50 uM

3 3D HNCO

Instrument

Varian INOVA - 600 MHz Rutgers

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.073 mM OR16.004, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	OR16.004	[U-100% 13C; U-100% 15N]	1.073 mM
2	Proteinase Inhibitors	natural abundance	1 na
3	NaN3	natural abundance	0.02 %
4	DTT	natural abundance	10 mM
5	CaCL2	natural abundance	5 mM
6	NaCL	natural abundance	200 mM
7	MES pH 6.5	natural abundance	20 mM
8	D2O	natural abundance	10 %
9	DSS	natural abundance	50 uM

4 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz Rutgers

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.073 mM OR16.004, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	OR16.004	[U-100% 13C; U-100% 15N]	1.073 mM
2	Proteinase Inhibitors	natural abundance	1 na
3	NaN3	natural abundance	0.02 %
4	DTT	natural abundance	10 mM
5	CaCL2	natural abundance	5 mM
6	NaCL	natural abundance	200 mM
7	MES pH 6.5	natural abundance	20 mM
8	D2O	natural abundance	10 %
9	DSS	natural abundance	50 uM

5 3D HNCACB

Instrument

Varian INOVA - 600 MHz Rutgers

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.073 mM OR16.004, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	OR16.004	[U-100% 13C; U-100% 15N]	1.073 mM
2	Proteinase Inhibitors	natural abundance	1 na
3	NaN3	natural abundance	0.02 %
4	DTT	natural abundance	10 mM
5	CaCL2	natural abundance	5 mM
6	NaCL	natural abundance	200 mM
7	MES pH 6.5	natural abundance	20 mM
8	D2O	natural abundance	10 %
9	DSS	natural abundance	50 uM

6 3D 1H-13C arom NOESY

Instrument

Bruker Avance - 800 MHz Rutgers

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.073 mM OR16.004, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	OR16.004	[U-100% 13C; U-100% 15N]	1.073 mM
2	Proteinase Inhibitors	natural abundance	1 na
3	NaN3	natural abundance	0.02 %
4	DTT	natural abundance	10 mM
5	CaCL2	natural abundance	5 mM
6	NaCL	natural abundance	200 mM
7	MES pH 6.5	natural abundance	20 mM
8	D2O	natural abundance	10 %
9	DSS	natural abundance	50 uM

7 3D simutaneous 13C-aromatic,13C-aliphatic,15N edited 1H-1H NOESY

Instrument

Bruker Avance - 800 MHz Rutgers

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.073 mM OR16.004, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	OR16.004	[U-100% 13C; U-100% 15N]	1.073 mM
2	Proteinase Inhibitors	natural abundance	1 na
3	NaN3	natural abundance	0.02 %
4	DTT	natural abundance	10 mM
5	CaCL2	natural abundance	5 mM
6	NaCL	natural abundance	200 mM
7	MES pH 6.5	natural abundance	20 mM
8	D2O	natural abundance	10 %
9	DSS	natural abundance	50 uM

8 3D C(CO)NH

Instrument

Varian INOVA - 600 MHz Rutgers

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.073 mM OR16.004, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	OR16.004	[U-100% 13C; U-100% 15N]	1.073 mM
2	Proteinase Inhibitors	natural abundance	1 na
3	NaN3	natural abundance	0.02 %
4	DTT	natural abundance	10 mM
5	CaCL2	natural abundance	5 mM
6	NaCL	natural abundance	200 mM
7	MES pH 6.5	natural abundance	20 mM
8	D2O	natural abundance	10 %
9	DSS	natural abundance	50 uM

9 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz Rutgers

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.073 mM OR16.004, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	OR16.004	[U-100% 13C; U-100% 15N]	1.073 mM
2	Proteinase Inhibitors	natural abundance	1 na
3	NaN3	natural abundance	0.02 %
4	DTT	natural abundance	10 mM
5	CaCL2	natural abundance	5 mM
6	NaCL	natural abundance	200 mM
7	MES pH 6.5	natural abundance	20 mM
8	D2O	natural abundance	10 %
9	DSS	natural abundance	50 uM

10 2D 1H-13C HSQC

Instrument

Varian INOVA - 600 MHz Rutgers

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.867 mM OR16.006, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
10	OR16.006	[U-100% 13C; U-100% 15N]	0.867 mM
11	Proteinase Inhibitors	natural abundance	1 na
12	NaN3	natural abundance	0.02 %
13	DTT	natural abundance	10 mM
14	CaCL2	natural abundance	5 mM
15	NaCL	natural abundance	200 mM
16	MES pH 6.5	natural abundance	20 mM
17	D2O	natural abundance	10 %
18	DSS	natural abundance	50 uM

11 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz UGA

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.859 mM OR16.013, 90% H2O/10% D2O alignment media peg or phage

#	Name	Isotope labeling	Concentration
19	OR16.013	[U-100% 13C; U-100% 15N]	0.859 mM
20	Proteinase Inhibitors	natural abundance	1 na
21	NaN3	natural abundance	0.02 %
22	DTT	natural abundance	10 mM
23	CaCL2	natural abundance	5 mM
24	NaCL	natural abundance	200 mM
25	MES pH 6.5	natural abundance	20 mM
26	D2O	natural abundance	10 %
27	DSS	natural abundance	50 uM

Keywords NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG), PSI-Biology, Protein NMR, Protein Structure Initiative, Structural Genomics, Target OR16

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Found 1 Document (0.712 seconds)

BMRB: 18558

Related entities 1. OR16, : 1 : 1 : 80 entities Detail

Experiments performed 11 experiments Detail

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