Solution NMR Structure of Ig like domain (805-892) of Obscurin-like protein 1 from Homo sapiens, Northeast Structural Genomics Consortium (NESG) Target HR8578K
SHMPVHIVDP REHVFVHAIT SECVMLACEV DREDAPVRWY KDGQEVEESD FVVLENEGPH RRLVLPATQP SDGGEFQCVA GDECAYFTVT I
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 95.5 % (971 of 1017) | 95.2 % (497 of 522) | 95.6 % (387 of 405) | 96.7 % (87 of 90) |
Backbone | 96.1 % (513 of 534) | 94.5 % (171 of 181) | 97.0 % (260 of 268) | 96.5 % (82 of 85) |
Sidechain | 94.2 % (536 of 569) | 93.8 % (320 of 341) | 94.6 % (211 of 223) | 100.0 % (5 of 5) |
Aromatic | 83.0 % (73 of 88) | 81.8 % (36 of 44) | 83.7 % (36 of 43) | 100.0 % (1 of 1) |
Methyl | 96.0 % (96 of 100) | 96.0 % (48 of 50) | 96.0 % (48 of 50) |
1. HR8578K
SHMPVHIVDP REHVFVHAIT SECVMLACEV DREDAPVRWY KDGQEVEESD FVVLENEGPH RRLVLPATQP SDGGEFQCVA GDECAYFTVT ISolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8578K.009, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR8578K.009 | [U-100% 13C; U-100% 15N] | 1.0 mM | |
2 | NaN3 | natural abundance | 0.02 % | |
3 | DTT | natural abundance | 10 mM | |
4 | CaCL2 | natural abundance | 5 mM | |
5 | NaCL | natural abundance | 100 mM | |
6 | Proteinase Inhibitors | natural abundance | 1 na | |
7 | MES pH 6.5 | natural abundance | 20 mM | |
8 | D2O | natural abundance | 10 % | |
9 | DSS | natural abundance | 50 uM | |
10 | H2O | natural abundance | 90 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8578K.009, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | HR8578K.010 | [U-5% 13C; U-100% 15N] | 1.0 mM | |
12 | NaN3 | natural abundance | 0.02 % | |
13 | DTT | natural abundance | 10 mM | |
14 | CaCL2 | natural abundance | 5 mM | |
15 | NaCL | natural abundance | 100 mM | |
16 | Proteinase Inhibitors | natural abundance | 1 na | |
17 | MES pH 6.5 | natural abundance | 20 mM | |
18 | D2O | natural abundance | 10 % | |
19 | DSS | natural abundance | 50 uM | |
20 | H2O | natural abundance | 90 % |
Solvent system 80% H2O/20% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.6 mM HR8578K.010, 80% H2O/20% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
21 | HR8578K.010 | [U-5% 13C; U-100% 15N] | 0.6 mM | |
22 | NaN3 | natural abundance | 0.02 % | |
23 | DTT | natural abundance | 10 mM | |
24 | CaCL2 | natural abundance | 5 mM | |
25 | NaCL | natural abundance | 100 mM | |
26 | Proteinase Inhibitors | natural abundance | 1 na | |
27 | MES pH 6.5 | natural abundance | 20 mM | |
28 | D2O | natural abundance | 10 % | |
29 | DSS | natural abundance | 50 uM | |
30 | PF1 Phage | natural abundance | 12.5 mg/mL | |
31 | H2O | natural abundance | 80 % |
Solvent system 80% H2O/20% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.6 mM HR8578K.010, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
32 | HR8578K.010 | [U-5% 13C; U-100% 15N] | 0.6 mM | |
33 | NaN3 | natural abundance | 0.02 % | |
34 | DTT | natural abundance | 10 mM | |
35 | CaCL2 | natural abundance | 5 mM | |
36 | NaCL | natural abundance | 100 mM | |
37 | Proteinase Inhibitors | natural abundance | 1 na | |
38 | MES pH 6.5 | natural abundance | 20 mM | |
39 | D2O | natural abundance | 10 % | |
40 | DSS | natural abundance | 50 uM | |
41 | PEG | natural abundance | 4 % | |
42 | H2O | natural abundance | 80 % |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
1H | DSS | methyl protons | 0.0 ppm | external | direct | 1.0 |
13C | DSS | methyl protons | 0.0 ppm | na | indirect | 0.2514495 |
15N | DSS | methyl protons | 0.0 ppm | na | indirect | 0.1013291 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
1H | DSS | methyl protons | 0.0 ppm | external | direct | 1.0 |
13C | DSS | methyl protons | 0.0 ppm | na | indirect | 0.2514495 |
15N | DSS | methyl protons | 0.0 ppm | na | indirect | 0.1013291 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
1H | DSS | methyl protons | 0.0 ppm | external | direct | 1.0 |
13C | DSS | methyl protons | 0.0 ppm | na | indirect | 0.2514495 |
15N | DSS | methyl protons | 0.0 ppm | na | indirect | 0.1013291 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
1H | DSS | methyl protons | 0.0 ppm | external | direct | 1.0 |
13C | DSS | methyl protons | 0.0 ppm | na | indirect | 0.2514495 |
15N | DSS | methyl protons | 0.0 ppm | na | indirect | 0.1013291 |
Varian INOVA - 750 MHz High-Field NMR facility at UB
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8578K.009, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR8578K.009 | [U-100% 13C; U-100% 15N] | 1.0 mM | |
2 | NaN3 | natural abundance | 0.02 % | |
3 | DTT | natural abundance | 10 mM | |
4 | CaCL2 | natural abundance | 5 mM | |
5 | NaCL | natural abundance | 100 mM | |
6 | Proteinase Inhibitors | natural abundance | 1 na | |
7 | MES pH 6.5 | natural abundance | 20 mM | |
8 | D2O | natural abundance | 10 % | |
9 | DSS | natural abundance | 50 uM | |
10 | H2O | natural abundance | 90 % |
Varian INOVA - 750 MHz High-Field NMR facility at UB
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8578K.009, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR8578K.009 | [U-100% 13C; U-100% 15N] | 1.0 mM | |
2 | NaN3 | natural abundance | 0.02 % | |
3 | DTT | natural abundance | 10 mM | |
4 | CaCL2 | natural abundance | 5 mM | |
5 | NaCL | natural abundance | 100 mM | |
6 | Proteinase Inhibitors | natural abundance | 1 na | |
7 | MES pH 6.5 | natural abundance | 20 mM | |
8 | D2O | natural abundance | 10 % | |
9 | DSS | natural abundance | 50 uM | |
10 | H2O | natural abundance | 90 % |
Varian INOVA - 750 MHz High-Field NMR facility at UB
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8578K.009, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR8578K.009 | [U-100% 13C; U-100% 15N] | 1.0 mM | |
2 | NaN3 | natural abundance | 0.02 % | |
3 | DTT | natural abundance | 10 mM | |
4 | CaCL2 | natural abundance | 5 mM | |
5 | NaCL | natural abundance | 100 mM | |
6 | Proteinase Inhibitors | natural abundance | 1 na | |
7 | MES pH 6.5 | natural abundance | 20 mM | |
8 | D2O | natural abundance | 10 % | |
9 | DSS | natural abundance | 50 uM | |
10 | H2O | natural abundance | 90 % |
Varian INOVA - 600 MHz High-Field NMR facility at UB
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8578K.009, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR8578K.009 | [U-100% 13C; U-100% 15N] | 1.0 mM | |
2 | NaN3 | natural abundance | 0.02 % | |
3 | DTT | natural abundance | 10 mM | |
4 | CaCL2 | natural abundance | 5 mM | |
5 | NaCL | natural abundance | 100 mM | |
6 | Proteinase Inhibitors | natural abundance | 1 na | |
7 | MES pH 6.5 | natural abundance | 20 mM | |
8 | D2O | natural abundance | 10 % | |
9 | DSS | natural abundance | 50 uM | |
10 | H2O | natural abundance | 90 % |
Varian INOVA - 600 MHz High-Field NMR facility at UB
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8578K.009, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR8578K.009 | [U-100% 13C; U-100% 15N] | 1.0 mM | |
2 | NaN3 | natural abundance | 0.02 % | |
3 | DTT | natural abundance | 10 mM | |
4 | CaCL2 | natural abundance | 5 mM | |
5 | NaCL | natural abundance | 100 mM | |
6 | Proteinase Inhibitors | natural abundance | 1 na | |
7 | MES pH 6.5 | natural abundance | 20 mM | |
8 | D2O | natural abundance | 10 % | |
9 | DSS | natural abundance | 50 uM | |
10 | H2O | natural abundance | 90 % |
Varian INOVA - 600 MHz High-Field NMR facility at UB
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8578K.009, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR8578K.009 | [U-100% 13C; U-100% 15N] | 1.0 mM | |
2 | NaN3 | natural abundance | 0.02 % | |
3 | DTT | natural abundance | 10 mM | |
4 | CaCL2 | natural abundance | 5 mM | |
5 | NaCL | natural abundance | 100 mM | |
6 | Proteinase Inhibitors | natural abundance | 1 na | |
7 | MES pH 6.5 | natural abundance | 20 mM | |
8 | D2O | natural abundance | 10 % | |
9 | DSS | natural abundance | 50 uM | |
10 | H2O | natural abundance | 90 % |
Varian INOVA - 750 MHz High-Field NMR facility at UB
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8578K.009, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR8578K.009 | [U-100% 13C; U-100% 15N] | 1.0 mM | |
2 | NaN3 | natural abundance | 0.02 % | |
3 | DTT | natural abundance | 10 mM | |
4 | CaCL2 | natural abundance | 5 mM | |
5 | NaCL | natural abundance | 100 mM | |
6 | Proteinase Inhibitors | natural abundance | 1 na | |
7 | MES pH 6.5 | natural abundance | 20 mM | |
8 | D2O | natural abundance | 10 % | |
9 | DSS | natural abundance | 50 uM | |
10 | H2O | natural abundance | 90 % |
Varian INOVA - 600 MHz High-Field NMR facility at UB
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8578K.009, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | HR8578K.010 | [U-5% 13C; U-100% 15N] | 1.0 mM | |
12 | NaN3 | natural abundance | 0.02 % | |
13 | DTT | natural abundance | 10 mM | |
14 | CaCL2 | natural abundance | 5 mM | |
15 | NaCL | natural abundance | 100 mM | |
16 | Proteinase Inhibitors | natural abundance | 1 na | |
17 | MES pH 6.5 | natural abundance | 20 mM | |
18 | D2O | natural abundance | 10 % | |
19 | DSS | natural abundance | 50 uM | |
20 | H2O | natural abundance | 90 % |
Varian INOVA - 600 MHz High-Field NMR facility at UB
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8578K.009, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR8578K.009 | [U-100% 13C; U-100% 15N] | 1.0 mM | |
2 | NaN3 | natural abundance | 0.02 % | |
3 | DTT | natural abundance | 10 mM | |
4 | CaCL2 | natural abundance | 5 mM | |
5 | NaCL | natural abundance | 100 mM | |
6 | Proteinase Inhibitors | natural abundance | 1 na | |
7 | MES pH 6.5 | natural abundance | 20 mM | |
8 | D2O | natural abundance | 10 % | |
9 | DSS | natural abundance | 50 uM | |
10 | H2O | natural abundance | 90 % |
Varian INOVA - 600 MHz High-Field NMR facility at UB
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8578K.009, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR8578K.009 | [U-100% 13C; U-100% 15N] | 1.0 mM | |
2 | NaN3 | natural abundance | 0.02 % | |
3 | DTT | natural abundance | 10 mM | |
4 | CaCL2 | natural abundance | 5 mM | |
5 | NaCL | natural abundance | 100 mM | |
6 | Proteinase Inhibitors | natural abundance | 1 na | |
7 | MES pH 6.5 | natural abundance | 20 mM | |
8 | D2O | natural abundance | 10 % | |
9 | DSS | natural abundance | 50 uM | |
10 | H2O | natural abundance | 90 % |
Varian INOVA - 600 MHz High-Field NMR facility at UB
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8578K.009, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR8578K.009 | [U-100% 13C; U-100% 15N] | 1.0 mM | |
2 | NaN3 | natural abundance | 0.02 % | |
3 | DTT | natural abundance | 10 mM | |
4 | CaCL2 | natural abundance | 5 mM | |
5 | NaCL | natural abundance | 100 mM | |
6 | Proteinase Inhibitors | natural abundance | 1 na | |
7 | MES pH 6.5 | natural abundance | 20 mM | |
8 | D2O | natural abundance | 10 % | |
9 | DSS | natural abundance | 50 uM | |
10 | H2O | natural abundance | 90 % |
Varian INOVA - 750 MHz High-Field NMR facility at UB
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8578K.009, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR8578K.009 | [U-100% 13C; U-100% 15N] | 1.0 mM | |
2 | NaN3 | natural abundance | 0.02 % | |
3 | DTT | natural abundance | 10 mM | |
4 | CaCL2 | natural abundance | 5 mM | |
5 | NaCL | natural abundance | 100 mM | |
6 | Proteinase Inhibitors | natural abundance | 1 na | |
7 | MES pH 6.5 | natural abundance | 20 mM | |
8 | D2O | natural abundance | 10 % | |
9 | DSS | natural abundance | 50 uM | |
10 | H2O | natural abundance | 90 % |
Varian INOVA - 600 MHz High-Field NMR facility at UB
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8578K.009, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR8578K.009 | [U-100% 13C; U-100% 15N] | 1.0 mM | |
2 | NaN3 | natural abundance | 0.02 % | |
3 | DTT | natural abundance | 10 mM | |
4 | CaCL2 | natural abundance | 5 mM | |
5 | NaCL | natural abundance | 100 mM | |
6 | Proteinase Inhibitors | natural abundance | 1 na | |
7 | MES pH 6.5 | natural abundance | 20 mM | |
8 | D2O | natural abundance | 10 % | |
9 | DSS | natural abundance | 50 uM | |
10 | H2O | natural abundance | 90 % |
Varian INOVA - 600 MHz High-Field NMR facility at UB
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8578K.009, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR8578K.009 | [U-100% 13C; U-100% 15N] | 1.0 mM | |
2 | NaN3 | natural abundance | 0.02 % | |
3 | DTT | natural abundance | 10 mM | |
4 | CaCL2 | natural abundance | 5 mM | |
5 | NaCL | natural abundance | 100 mM | |
6 | Proteinase Inhibitors | natural abundance | 1 na | |
7 | MES pH 6.5 | natural abundance | 20 mM | |
8 | D2O | natural abundance | 10 % | |
9 | DSS | natural abundance | 50 uM | |
10 | H2O | natural abundance | 90 % |
Varian INOVA - 600 MHz High-Field NMR facility at UB
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8578K.009, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR8578K.009 | [U-100% 13C; U-100% 15N] | 1.0 mM | |
2 | NaN3 | natural abundance | 0.02 % | |
3 | DTT | natural abundance | 10 mM | |
4 | CaCL2 | natural abundance | 5 mM | |
5 | NaCL | natural abundance | 100 mM | |
6 | Proteinase Inhibitors | natural abundance | 1 na | |
7 | MES pH 6.5 | natural abundance | 20 mM | |
8 | D2O | natural abundance | 10 % | |
9 | DSS | natural abundance | 50 uM | |
10 | H2O | natural abundance | 90 % |
Varian INOVA - 600 MHz High-Field NMR facility at UB
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8578K.009, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR8578K.009 | [U-100% 13C; U-100% 15N] | 1.0 mM | |
2 | NaN3 | natural abundance | 0.02 % | |
3 | DTT | natural abundance | 10 mM | |
4 | CaCL2 | natural abundance | 5 mM | |
5 | NaCL | natural abundance | 100 mM | |
6 | Proteinase Inhibitors | natural abundance | 1 na | |
7 | MES pH 6.5 | natural abundance | 20 mM | |
8 | D2O | natural abundance | 10 % | |
9 | DSS | natural abundance | 50 uM | |
10 | H2O | natural abundance | 90 % |
Varian INOVA - 600 MHz Univ. of Georgia
State anisotropic, Solvent system 80% H2O/20% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.6 mM HR8578K.010, 80% H2O/20% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
21 | HR8578K.010 | [U-5% 13C; U-100% 15N] | 0.6 mM | |
22 | NaN3 | natural abundance | 0.02 % | |
23 | DTT | natural abundance | 10 mM | |
24 | CaCL2 | natural abundance | 5 mM | |
25 | NaCL | natural abundance | 100 mM | |
26 | Proteinase Inhibitors | natural abundance | 1 na | |
27 | MES pH 6.5 | natural abundance | 20 mM | |
28 | D2O | natural abundance | 10 % | |
29 | DSS | natural abundance | 50 uM | |
30 | PF1 Phage | natural abundance | 12.5 mg/mL | |
31 | H2O | natural abundance | 80 % |
Varian INOVA - 600 MHz Univ. of Georgia
State anisotropic, Solvent system 80% H2O/20% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.6 mM HR8578K.010, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
32 | HR8578K.010 | [U-5% 13C; U-100% 15N] | 0.6 mM | |
33 | NaN3 | natural abundance | 0.02 % | |
34 | DTT | natural abundance | 10 mM | |
35 | CaCL2 | natural abundance | 5 mM | |
36 | NaCL | natural abundance | 100 mM | |
37 | Proteinase Inhibitors | natural abundance | 1 na | |
38 | MES pH 6.5 | natural abundance | 20 mM | |
39 | D2O | natural abundance | 10 % | |
40 | DSS | natural abundance | 50 uM | |
41 | PEG | natural abundance | 4 % | |
42 | H2O | natural abundance | 80 % |
Varian INOVA - 600 MHz Univ. of Georgia
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8578K.009, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | HR8578K.010 | [U-5% 13C; U-100% 15N] | 1.0 mM | |
12 | NaN3 | natural abundance | 0.02 % | |
13 | DTT | natural abundance | 10 mM | |
14 | CaCL2 | natural abundance | 5 mM | |
15 | NaCL | natural abundance | 100 mM | |
16 | Proteinase Inhibitors | natural abundance | 1 na | |
17 | MES pH 6.5 | natural abundance | 20 mM | |
18 | D2O | natural abundance | 10 % | |
19 | DSS | natural abundance | 50 uM | |
20 | H2O | natural abundance | 90 % |
Properties
Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints, RDC restraints | combined_18563_2lvc.nef |
Input source #2: Coordindates | 2lvc.cif |
Diamagnetism of the molecular assembly | True (excluding Oxygen atoms) |
Whether the assembly has a disulfide bond | None |
Whether the assembly has a other bond | None |
Whether the assembly contains a cyclic polymer | None |
Overall data processing status | Warning |
Disulfide bonds
NoneOther bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)
NoneNon-standard residues
NoneSequence alignments
0--------10--------20--------30--------40--------50--------60--------70--------80--------90 SHMPVHIVDPREHVFVHAITSECVMLACEVDREDAPVRWYKDGQEVEESDFVVLENEGPHRRLVLPATQPSDGGEFQCVAGDECAYFTVTI ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| SHMPVHIVDPREHVFVHAITSECVMLACEVDREDAPVRWYKDGQEVEESDFVVLENEGPHRRLVLPATQPSDGGEFQCVAGDECAYFTVTI --------10--------20--------30--------40--------50--------60--------70--------80--------90-
Chain assignments
Entity_assembly_ID (NMR) | Auth_asym_ID (model) | Length | Unmapped | Conflict | Sequence coverage (%) |
---|---|---|---|---|---|
A | A | 91 | 0 | 0 | 100.0 |
Content subtype: combined_18563_2lvc.nef
Assigned chemical shifts
0--------10--------20--------30--------40--------50--------60--------70--------80--------90 SHMPVHIVDPREHVFVHAITSECVMLACEVDREDAPVRWYKDGQEVEESDFVVLENEGPHRRLVLPATQPSDGGEFQCVAGDECAYFTVTI |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| .HMPVHIVDPREHVFVHAITSECVMLACEVDREDAPVRWYKDGQEVEESDFVVLENEGPHRRLVLPATQPSDGGEFQCVAGDECAYFTVTI
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 522 | 508 | 97.3 |
13C chemical shifts | 405 | 393 | 97.0 |
15N chemical shifts | 95 | 92 | 96.8 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 181 | 177 | 97.8 |
13C chemical shifts | 182 | 178 | 97.8 |
15N chemical shifts | 85 | 82 | 96.5 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 341 | 331 | 97.1 |
13C chemical shifts | 223 | 215 | 96.4 |
15N chemical shifts | 10 | 10 | 100.0 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 52 | 52 | 100.0 |
13C chemical shifts | 52 | 52 | 100.0 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 44 | 36 | 81.8 |
13C chemical shifts | 43 | 36 | 83.7 |
15N chemical shifts | 1 | 1 | 100.0 |
Distance restraints
0--------10--------20--------30--------40--------50--------60--------70--------80--------90 SHMPVHIVDPREHVFVHAITSECVMLACEVDREDAPVRWYKDGQEVEESDFVVLENEGPHRRLVLPATQPSDGGEFQCVAGDECAYFTVTI ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ..MPVHIVDPREHVFVHAITSECVMLACEVDREDAPVRWYKDGQEVEESDFVVLENEGPHRRLVLPATQPSDGGEFQCVAGDECAYFTVTI
Dihedral angle restraints
0--------10--------20--------30--------40--------50--------60--------70--------80--------90 SHMPVHIVDPREHVFVHAITSECVMLACEVDREDAPVRWYKDGQEVEESDFVVLENEGPHRRLVLPATQPSDGGEFQCVAGDECAYFTVTI ||||||| ||||||||| |||||| |||||| ||||| ||||||| |||||||| ............HVFVHAI..ECVMLACEV.....PVRWYK..........VVLENE...RRLVL........GEFQCVA..ECAYFTVT 0--------10--------20--------30--------40--------50--------60--------70--------80---------
RDC restraints
0--------10--------20--------30--------40--------50--------60--------70--------80--------90 SHMPVHIVDPREHVFVHAITSECVMLACEVDREDAPVRWYKDGQEVEESDFVVLENEGPHRRLVLPATQPSDGGEFQCVAGDECAYFTVTI | || |||||||||| | | |||||||||| || ||||||||||||||||||| |||||| || ||||||||||| ||||||||| ....V..VD.REHVFVHAIT.E.V.LACEVDREDA.VR.YKDGQEVEESDFVVLENEG.HRRLVL.AT..SDGGEFQCVAG.ECAYFTVTI
0--------10--------20--------30--------40--------50--------60--------70--------80--------90 SHMPVHIVDPREHVFVHAITSECVMLACEVDREDAPVRWYKDGQEVEESDFVVLENEGPHRRLVLPATQPSDGGEFQCVAGDECAYFTVTI | || |||||||||| | |||||||||||| || ||||||||||||||||||| || ||| || |||||||||| ||||||||| ....V..VD.REHVFVHAIT.E.VMLACEVDREDA.VR.YKDGQEVEESDFVVLENEG.HR.LVL.AT..SDGGEFQCVA..ECAYFTVTI