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BMRB: 18565

2N9V

Backbone and side-chain resonance assignments of the membrane localization domain from Pasteurella multocida toxin

Authors

Brothers, M.C., Geissler, B., Hisao, G.S., Satchell, K.J., Wilson, B.A., Rienstra, C.M.

Assembly

MLD PMT

Entity

1. MLD PMT (polymer, Thiol state: not present), 90 monomers, 10511.91 Da Detail

MGVWTPEVLK ARASVIGKPI GESYKRILAK LQRIHNSNIL DERQGLMHEL MELIDLYEES QPSSERLNAF RELRTQLEKA LGLEHHHHHH

Formula weight

10511.91 Da

Source organism

Pasteurella multocida

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.7 %, Completeness: 85.1 %, Completeness (bb): 94.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.1 % (925 of 1087)	83.0 % (475 of 572)	87.6 % (368 of 420)	86.3 % (82 of 95)
Backbone	94.4 % (504 of 534)	94.0 % (171 of 182)	94.7 % (251 of 265)	94.3 % (82 of 87)
Sidechain	78.7 % (502 of 638)	77.9 % (304 of 390)	82.5 % (198 of 240)	0.0 % (0 of 8)
Aromatic	4.3 % (3 of 70)	8.6 % (3 of 35)	0.0 % (0 of 34)	0.0 % (0 of 1)
Methyl	97.1 % (99 of 102)	96.1 % (49 of 51)	98.0 % (50 of 51)

1. MLD PMT

MGVWTPEVLK ARASVIGKPI GESYKRILAK LQRIHNSNIL DERQGLMHEL MELIDLYEES QPSSERLNAF RELRTQLEKA LGLEHHHHHH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	MLD PMT	[U-99% 13C; U-99% 15N]	1 mM
2	Bis-Tris	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	EDTA	natural abundance	1 mM
5	sodium azide	natural abundance	0.01 mM
6	DSS	natural abundance	0.01 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.39 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2N9V, Strand ID: A Detail

Release date

2014-02-10

Citation

Backbone and side-chain resonance assignments of the membrane localization domain from Pasteurella multocida toxin
Brothers, M.C., Geissler, B., Hisao, G.S., Satchell, K.J.F., Wilson, B.A., Rienstra, C.M.
Biomol. NMR Assign. (2014), 8, 221-224, PubMed 23765284 , DOI 10.1007/s12104-013-9487-1 , Abstract

Related entities 1. MLD PMT, : 1 : 4 : 14 entities Detail

Interaction partners 1. MLD PMT, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC