Solution structure of the soluble domain of MmpS4 from Mycobacterium tuberculosis
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 75.9 % (746 of 983) | 73.2 % (366 of 500) | 78.1 % (303 of 388) | 81.1 % (77 of 95) |
Backbone | 87.2 % (464 of 532) | 84.7 % (155 of 183) | 88.2 % (232 of 263) | 89.5 % (77 of 86) |
Sidechain | 67.4 % (360 of 534) | 66.6 % (211 of 317) | 71.6 % (149 of 208) | 0.0 % (0 of 9) |
Aromatic | 2.8 % (2 of 72) | 0.0 % (0 of 36) | 5.7 % (2 of 35) | 0.0 % (0 of 1) |
Methyl | 82.1 % (92 of 112) | 82.1 % (46 of 56) | 82.1 % (46 of 56) |
1. soluble domain of MmpS4
MHLTYEIFGP PGTVADISYF DVNSEPQRVD GAVLPWSLHI TTNDAAVMGN IVAQGNSDSI GCRITVDGKV RAERVSNEVN AYTYCLVKSASolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | MmpS4 | [U-100% 13C; U-100% 15N] | 0.2 ~ 0.5 mM | |
2 | DTT | natural abundance | 2 mM | |
3 | sodium phosphate | natural abundance | 50 mM | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | MmpS4 | [U-100% 15N] | 0.2 ~ 0.5 mM | |
7 | DTT | natural abundance | 2 mM | |
8 | sodium phosphate | natural abundance | 50 mM | |
9 | H2O | natural abundance | 90 % | |
10 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | MmpS4 | [U-100% 13C; U-100% 15N] | 0.2 ~ 0.5 mM | |
12 | DTT | natural abundance | 2 mM | |
13 | sodium phosphate | natural abundance | 50 mM | |
14 | D2O | natural abundance | 100 % |