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4B2R

Solution structure of CCP modules 10-11 of complement factor H

Authors

Makou, E., Mertens, H.D.T., Maciejewski, M., Soares, D.C., Matis, I., Schmidt, C.Q., Herbert, A.P., Svergun, D.I., Barlow, P.N.

Assembly

Solution structure of CCP modules 10-11 of complement factor H

Entity

1. Solution structure of CCP modules 10-11 of complement factor H (polymer, Thiol state: all disulfide bound), 126 monomers, 14168.11 Da Detail

EAAGERECEL PKIDVHLVPD RKKDQYKVGE VLKFSCKPGF TIVGPNSVQC YHFGLSPDLP ICKEQVQSCG PPPELLNGNV KEKTKEEYGH SEVVEYYCNP RFLMKGPNKI QCVDGEWTTL PVCIVE

Formula weight

14168.11 Da

Entity Connection

disulfide 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS8:SG	1:CYS50:SG
2	disulfide	sing	1:CYS98:SG	1:CYS123:SG
3	disulfide	sing	1:CYS69:SG	1:CYS112:SG
4	disulfide	sing	1:CYS36:SG	1:CYS62:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.4 %, Completeness (bb): 98.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.4 % (1433 of 1486)	95.8 % (750 of 783)	96.9 % (560 of 578)	98.4 % (123 of 125)
Backbone	98.5 % (721 of 732)	97.2 % (243 of 250)	99.5 % (366 of 368)	98.2 % (112 of 114)
Sidechain	95.2 % (828 of 870)	95.1 % (507 of 533)	95.1 % (310 of 326)	100.0 % (11 of 11)
Aromatic	88.5 % (92 of 104)	88.5 % (46 of 52)	88.2 % (45 of 51)	100.0 % (1 of 1)
Methyl	93.3 % (112 of 120)	96.7 % (58 of 60)	90.0 % (54 of 60)

1. COMPLEMENT FACTOR H

EAAGERECEL PKIDVHLVPD RKKDQYKVGE VLKFSCKPGF TIVGPNSVQC YHFGLSPDLP ICKEQVQSCG PPPELLNGNV KEKTKEEYGH SEVVEYYCNP RFLMKGPNKI QCVDGEWTTL PVCIVE

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.700, Details FH10-11 0.000400 M

#	Name	Isotope labeling	Concentration
1	COMPLEMENT FACTOR H	[U-13C; U-15N]	0.4 mM
2	potassium phosphate buffer	natural abundance	20 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.700, Details FH10-11 0.000540 M

#	Name	Isotope labeling	Concentration
5	COMPLEMENT FACTOR H	[U-13C; U-15N]	0.54 mM
6	potassium phosphate buffer	natural abundance	20 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.700, Details FH10-11 0.000150 M

#	Name	Isotope labeling	Concentration
9	COMPLEMENT FACTOR H	[U-13C; U-15N]	0.15 mM
10	potassium phosphate buffer	natural abundance	20 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.700, Details FH10-11 0.000500 M

#	Name	Isotope labeling	Concentration
13	COMPLEMENT FACTOR H	[U-13C; U-15N]	0.5 mM
14	potassium phosphate buffer	natural abundance	20 mM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.44 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.44 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	internal	indirect	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.83 ppm, Outliers: 2 Detail

Release date

2012-10-17

Citation

Solution structure of CCP modules 10-12 illuminates functional architecture of the complement regulator, factor H
Makou, E., Mertens, H.D.T., Maciejewski, M., Soares, D.C., Matis, I., Schmidt, C.Q., Herbert, A.P., Svergun, D.I., Barlow, P.N.
J. Mol. Biol. (2012), 424, 295-312, PubMed 23017427 , DOI 10.1016/j.jmb.2012.09.013 , Abstract

Related entities 1. Solution structure of CCP modules 10-11 of complement factor H, : 1 : 6 : 393 entities Detail

Interaction partners 1. Solution structure of CCP modules 10-11 of complement factor H, : 17 interactors Detail

More details for 17 interactors identified by 13 citations

Experiments performed 15 experiments Detail

1 CbCANH