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Found 1 Document (1.361 seconds)

BMRB: 18614

2LW9

NMR solution structure of Myo10 anti-CC

Authors

Ye, F., Lu, Q., Zhang, M.

Assembly

Myo10 anti-CC

Entity

1. Myo10 anti-CC (polymer, Thiol state: not present), 51 monomers, 6337.987 × 2 Da Detail

ENKQVEEILR LEKEIEDLQR MKEQQELSLT EASLQKLQER RDQELRRLEE E

Total weight

12675.974 Da

Max. entity weight

6337.987 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 79.1 %, Completeness (bb): 80.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	79.1 % (512 of 647)	82.7 % (291 of 352)	72.0 % (170 of 236)	86.4 % (51 of 59)
Backbone	80.1 % (245 of 306)	92.2 % (94 of 102)	65.4 % (100 of 153)	100.0 % (51 of 51)
Sidechain	79.6 % (312 of 392)	78.8 % (197 of 250)	85.8 % (115 of 134)	0.0 % (0 of 8)
Methyl	84.6 % (44 of 52)	84.6 % (22 of 26)	84.6 % (22 of 26)

1. entity

ENKQVEEILR LEKEIEDLQR MKEQQELSLT EASLQKLQER RDQELRRLEE E

Show sample condition

Sample

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Myo10_anti-CC	[U-100% 13C; U-100% 15N]		0.8 mM
2	D2O	natural abundance		100 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2LW9, Strand ID: A, B Detail

Release date

2012-09-23

Citation

Antiparallel coiled-coil-mediated dimerization of myosin X
Lu, Q., Ye, F., Wei, Z., Wen, Z., Zhang, M.
Proc. Natl. Acad. Sci. U. S. A. (2012), 109, 17388-17393, PubMed 23012428 , DOI 10.1073/pnas.1208642109 , Abstract

Related entities 1. Myo10 anti-CC, : 1 : 2 : 12 entities Detail

Interaction partners 1. Myo10 anti-CC, : 16 interactors Detail

More details for 16 interactors identified by 8 citations

Experiments performed 6 experiments Detail

NMR combined restraints 5 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_18614_2lw9.nef
Input source #2: Coordindates	2lw9.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50-
ENKQVEEILRLEKEIEDLQRMKEQQELSLTEASLQKLQERRDQELRRLEEE
|||||||||||||||||||||||||||||||||||||||||||||||||||
ENKQVEEILRLEKEIEDLQRMKEQQELSLTEASLQKLQERRDQELRRLEEE

Entity_assembly_ID (NMR): B vs Auth_asym_ID (model): B

------60--------70--------80--------90-------100---
ENKQVEEILRLEKEIEDLQRMKEQQELSLTEASLQKLQERRDQELRRLEEE
|||||||||||||||||||||||||||||||||||||||||||||||||||
ENKQVEEILRLEKEIEDLQRMKEQQELSLTEASLQKLQERRDQELRRLEEE
--------10--------20--------30--------40--------50-

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	51	0	0	100.0
B	B	51	0	0	100.0

Content subtype: combined_18614_2lw9.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	489		100.0 (chain: A, length: 51)
1	Distance restraints	CNS/XPLOR_distance_constraints_4	OK	4435 (1)	noe	84.3 (chain: A, length: 51), 84.3 (chain: B, length: 51)
2	Distance restraints	CNS/XPLOR_distance_constraints_2	OK	104 (1)	hbond	66.7 (chain: A, length: 51), 66.7 (chain: B, length: 51)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_3	OK	164 (164)	.	84.3 (chain: A, length: 51), 84.3 (chain: B, length: 51)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 51, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 283
  - ¹³C chemical shifts: 155
  - ¹⁵N chemical shifts: 51
- Completeness of assignments
  - Entity_assemble_ID: A
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	352	282	80.1
¹³C chemical shifts	236	155	65.7
¹⁵N chemical shifts	65	51	78.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	102	93	91.2
¹³C chemical shifts	102	43	42.2
¹⁵N chemical shifts	51	51	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	250	189	75.6
¹³C chemical shifts	134	112	83.6
¹⁵N chemical shifts	14	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	27	23	85.2
¹³C chemical shifts	27	23	85.2

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_4
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 51, Sequence coverage: 84.3%
  - Entity_assembly_ID: B, Chain length: 51, Sequence coverage: 84.3%
- Total number of restraints: 4387
- Weight of restraints: 1.0 (4387)
- Potential type of restraints: square-well-parabolic (4387)
- Range of distance target values: 2.35, 7.75 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
  - Chain_ID: B
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_2
  - Status: OK
  - Experiment type: hbond
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 51, Sequence coverage: 66.7%
    - Entity_assembly_ID: B, Chain length: 51, Sequence coverage: 66.7%
  - Total number of restraints: 104
  - Weight of restraints: 1.0 (104)
  - Potential type of restraints: square-well-parabolic (104)
  - Range of distance target values: 2.0, 2.75 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
    - Chain_ID: B
  - Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_3
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 51, Sequence coverage: 84.3%
  - Entity_assembly_ID: B, Chain length: 51, Sequence coverage: 84.3%
- Total number of restraints: 164
- Total number of restraints per polymer type: 164
- Weight of restraints: 1.0 (164)
- Potential type of restraints: square-well-parabolic (164)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A
  - Chain_ID: B

Keywords Myo10 anti-CC

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Found 1 Document (1.361 seconds)

BMRB: 18614

Sample

Related entities 1. Myo10 anti-CC, : 1 : 2 : 12 entities Detail

Interaction partners 1. Myo10 anti-CC, : 16 interactors Detail

Experiments performed 6 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

NMR combined restraints 5 contents Detail