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NMR solution structure of Myo10 anti-CC
Authors
Ye, F., Lu, Q., Zhang, M.
Assembly
Myo10 anti-CC
Entity
1. Myo10 anti-CC (polymer, Thiol state: not present), 51 monomers, 6337.987 × 2 Da Detail

ENKQVEEILR LEKEIEDLQR MKEQQELSLT EASLQKLQER RDQELRRLEE E


Total weight
12675.974 Da
Max. entity weight
6337.987 Da
Source organism
Escherichia coli
Exptl. method
solution NMR
Refine. method
DGSA-distance geometry simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 79.1 %, Completeness (bb): 80.1 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All79.1 % (512 of 647)82.7 % (291 of 352)72.0 % (170 of 236)86.4 % (51 of 59)
Backbone80.1 % (245 of 306)92.2 % (94 of 102)65.4 % (100 of 153)100.0 % (51 of 51)
Sidechain79.6 % (312 of 392)78.8 % (197 of 250)85.8 % (115 of 134) 0.0 % (0 of 8)
Methyl84.6 % (44 of 52)84.6 % (22 of 26)84.6 % (22 of 26)

1. entity

ENKQVEEILR LEKEIEDLQR MKEQQELSLT EASLQKLQER RDQELRRLEE E

Sample

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5


#NameIsotope labelingTypeConcentration
1Myo10_anti-CC[U-100% 13C; U-100% 15N]0.8 mM
2D2Onatural abundance100 %

Protein Blocks Logo
Calculated from 20 models in PDB: 2LW9, Strand ID: A, B Detail


Release date
2012-09-23
Citation
Antiparallel coiled-coil-mediated dimerization of myosin X
Lu, Q., Ye, F., Wei, Z., Wen, Z., Zhang, M.
Proc. Natl. Acad. Sci. U. S. A. (2012), 109, 17388-17393, PubMed 23012428 , DOI 10.1073/pnas.1208642109 ,
Related entities 1. Myo10 anti-CC, : 1 : 2 : 12 entities Detail
Interaction partners 1. Myo10 anti-CC, : 16 interactors Detail
Experiments performed 6 experiments Detail
NMR combined restraints 5 contents Detail
Keywords Myo10 anti-CC