Conformational ensemble for the G8A mutant of the influenza hemagglutinin fusion peptide
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 32.2 % (110 of 342) | 31.3 % (55 of 176) | 24.6 % (33 of 134) | 68.8 % (22 of 32) |
Backbone | 57.8 % (104 of 180) | 77.9 % (53 of 68) | 35.4 % (29 of 82) | 73.3 % (22 of 30) |
Sidechain | 4.3 % (8 of 184) | 1.9 % (2 of 108) | 8.1 % (6 of 74) | 0.0 % (0 of 2) |
Aromatic | 0.0 % (0 of 52) | 0.0 % (0 of 26) | 0.0 % (0 of 24) | 0.0 % (0 of 2) |
Methyl | 8.3 % (2 of 24) | 8.3 % (1 of 12) | 8.3 % (1 of 12) |
1. Hemagglutinin Fusion Peptide G8A mutant
GLFGAIAAFI EGGWTGMIDG WYGSGKKKKDSolvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 305.0 (±0.1) K, pH 7.3 (±0.1), Details pH 7.3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Hemagglutinin Fusion Peptide G8A mutant | [U-100% 13C; U-100% 15N] | 0.3 ~ 0.6 (±0.1) mM | |
2 | TRIS | natural abundance | 25 mM | |
3 | DPC | [U-100% 2H] | 130.0 ~ 160.0 mM | |
4 | D2O | natural abundance | 7 % | |
5 | H2O | natural abundance | 93 % |